摘要
目的采用HPLC法测定大黄中番泻苷A和番泻苷B的含量。方法色谱柱E1718897 Hypersil C18(4.6mm×250mm,25μm),流动相为四氢呋喃-水-醋酸(15∶85∶1.5),流速为0.8mL/min。结果番泻苷A的含量在0.176～1.76g/L范围内呈良好的线性关系(r=0.999 5),番泻苷B的含量在0.12～1.2g/L范围内呈良好的线性关系(r=0.999 5),平均加样回收率番泻苷A为100.44%(RSD=2.44%),番泻苷B为101.37%(RSD=2.28%)。结论该方法简便、准确、重复性好,可用于大黄药材中番泻苷A、B含量的测定。
Objective To establish a method for determining sennoside A and sennoside B in Rhubarbs by HPLC. Methods sennoside A and sennoside B was analysed by using Hypersil C18 column（4.6 mm× 250 mm, 25 μm） with the mobile phase of tetrahydrofuran-water-acetic acid（15 ： 85 ： 1.5） at the flow rate of 0.8 mL/min, and the detective wavelength was 350 nm. Results The linear range of sennoside A was from 0. 176 g/L to 1.76 g/L （r = 0.999 5）, and the linear range of sennoside B was from 0.12 g/L to 1.2 g/L （r =0. 999 5）. The average recoveries were 100.44% for sennoside A （ RSD = 2.44%） and 101.37% for sennoside B （ RSD = 2.28% ）. Conclusion The method is simple, accurate and suitable for the determination of sennoside A and sennoside B in Rhubarb.
出处
《河南大学学报（医学版）》
CAS
2013年第2期105-108,共4页
Journal of Henan University:Medical Science
