摘要
目的:研究口虾蛄提取物(extract of Oratosquilla,EOS)对人低分化鼻咽癌细胞株CNE-2的迁移及体外血管生成拟态的影响。方法:用不同浓度(0 mg/L、125 mg/L、250 mg/L、500 mg/L)EOS处理CNE-2细胞24 h后,创伤修复实验检测细胞迁移能力;通过Matrigel三维细胞培养观察CNE-2细胞形成类血管网状结构的能力及其特点;体外管道形成抑制实验检测不同浓度EOS对CNE-2细胞管道形成能力的影响;Western blotting法检测不同浓度EOS对CNE-2细胞fascin 1和血管内皮生长因子(VEGF)蛋白表达的影响。结果:EOS可以显著降低CNE-2细胞的迁移能力,与对照组比较差异有统计学意义(P<0.01);CNE-2细胞在Matrigel上培养能形成类似血管的网状样结构;EOS能以剂量依赖方式抑制CNE-2细胞体外管道形成的数量(P<0.01);EOS能抑制CNE-2细胞中fas-cin 1和VEGF蛋白的表达(P<0.01),且其管状结构数量与2种蛋白变化趋势呈正相关(P<0.05)。结论:CNE-2细胞具有血管生成拟态的能力;EOS能够抑制CNE-2细胞的迁移和体外血管生成拟态的能力,其机制可能与下调fascin 1和VEGF蛋白的表达有关。
AIM： To study the inhibitory effect of the extract of OratosquiUa （EOS） on the migration and vas- culogenic mimicry in human poorly differentiated nasopharyngeal carcinoma cell line CNE-2. METHODS： CNE-2 cells were cultured in the medium with different concentrations of EOS （0 rag/L, 125 rag/L, 250 mg/L and 500 mg/L）. The migration of CNE-2 cells and the formation of tube-like structures （TLSs） by CNE-2 cells were determined with wound healing assay and in vitro anti-angiogenesis test, respectively. The formation of TLSs by CNE-2 ceils and their structural characteristics were observed by anti-angiogenesis test on the Matrigel. The protein expression of fascin 1 and vascular en- dothelial growth factor （VEGF） was detected by Western blotting. RESULTS： Compared with control group, EOS signifi- cantly decreased the migration velocity of CNE-2 cells in a dose-dependent manner. CNE-2 cells formed TLSs on the Matri- gel, and the formation of TLSs by CNE-2 cells was inhibited by EOS in a dose-dependent manner. The expression of fascin 1 and VEGF in CNE-2 cells was also decreased after treatment with EOS. A positive correlation between the expression of fascin 1/VEGF and the formation of TLSs by CNE-2 cells was observed. CONCLUSION： CNE-2 cells form TLSs on the Matrigel, and EOS inhibits the migration and vasculogenic mimicry of CNE-2 cells, which are related with down-regulating the expression of fascin 1 and VEGF in CNE-2 ceils .
出处
《中国病理生理杂志》
CAS
CSCD
北大核心
2013年第6期1025-1028,共4页
Chinese Journal of Pathophysiology
基金
广东省医学科研基金资助项目(No.B2011235)
广东省中医药管理局资助课题(No.20122082)
东莞市高等院校科研计划(No.200910815267)
关键词
口虾蛄
鼻咽肿瘤
血管生成拟态
Oratosquilla
Nasopharyngeal neoplasms
Vasculogenic mimicry
