摘要
纯种的分离培养是微藻产品开发、规模培养和科学研究的基础。微藻易受其他生物污染,又无法低温保存,导致其分离培养面临困难。比较了不同浓度的3种农药和3种抗生素分别对微茫藻18A8,小球藻CE14和亚心形扁藻,小新月菱形藻生长的影响,确定它们用于藻种分离的最佳浓度为百菌清25 mg/L、水合霉素25 mg/L、绿邦200 mg/L、卡那霉素25 mg/L、氨苄西林25 mg/L和制菌霉素1.25 mg/L。采集含有微藻的淡水、海水水样先后培养在混合添加了3种适宜浓度的农药和抗生素的培养基,最终抑制了微藻中其他真菌和细菌的生长,实现了微藻的藻种纯化。
The isolation and cultivation of species are the basis of microalgae products exploring and scientific research. The microalgae is susceptible contamination to other microorganism when cultured, and can not be cryopreserved. The microalgae species isolation faced difficulties. This study compared different concentrations of the three pesticides and antibiotics effects on Micractinium pusillum 18A8, Heynigia riparia CE14-2, Platymonas subcordiformis and Phaeodactylum tricornutum. The optimum concentrations of these pesticides and antibiotics for mireoalgae were determined as 25 mg/L for chlorothalonil, 25 mg/L for water synthomycin, 200 mg/L for Lv Bang, 25 mg/L for Ampicillin, 25 mg/L kanamycin and 1.25 mg/L for nysfungin. The fresh water and seawater containing microalgae were collected from nature, cuhrued in medium added three appropriate concentrations pesticides and antibiotics, and eventually the other fungi and bacteria were inhibited, the microalgae single species were obtained.
出处
《广东农业科学》
CAS
CSCD
北大核心
2013年第10期86-88,F0004,共4页
Guangdong Agricultural Sciences
基金
国家自然科学基金(31000117)
中央级公益性科研院所基本科研业务费专项(CATAS-ITBB130305
CATAS-ITBB110507)
关键词
微藻
藻种纯化
敏感性
农药
抗生素
microalgae
single-species culture
sensitivity
growth rate
