摘要
目的:观察复合纤维蛋白的多孔自凝固磷酸钙的理学性能及体内血管化情况,探讨其各组份对材料生物学特性的影响,为其临床应用提供实验数据。方法:1)采用凝固时间测定和电镜观察材料表面和断面等方法对复合支架材料构造和理学性能进行分析;2)将24只新西兰白兔随机分为3组,每组8只,分别在每只实验兔腰背筋膜下植入1:1、CPC两种比例材料各一枚。术后2、4、8周进行取材,对材料及其周围组织进行组织学观察、微血管情况定量分析。结果:1)CPC/FG复合支架材料以1:1(g/ml)混合后,与单纯的CPC相比,初凝时间延长,而终凝时间没有明显的统计学差异;电镜观察发现多孔自凝固磷酸钙复合了纤维蛋白胶之后,纤维蛋白胶分布均匀贯穿多孔自凝固磷酸钙晶体之间,并将其紧密相连;2)术后2周材料外周可见幼稚的微血管。1:1组高于CPC组(P<0.05)。4周微血管密度达到高峰,相对于2周时有明显差异。1:1组高于CPC组(P<0.05)。8周时微血管密度相对4周没有显著差异。各组微血管密度与4周时没有明显变化(P>0.05)。结论:复合支架材料具有合适的凝固时间和较好的结构,纤维蛋白胶及其降解产物影响复合材料在体内的微血管密度,使复合材料血管化能力提高,其可作为细胞载体和骨缺损修复支架材料应用于临床和实验中。
Objective： To investigate physical performance and microvessel density of porous calcium phosphate composite and fibrin glue,to discuss the various components of material biological characteristics,to provide the experimental foundation for the clinical application.Methods： 1） Clotting assay and electron microscopy（sem） method is used to analyze composite scaffold structure and physical performance;2） Twenty-four Zealand rabbits were divided into 3 groups（n=8） randomly.CPC and CPC/FG of 1：1 were implanted separately into the facia lumbodorsalis of rabbits from different groups.In order to observe the vascularization by means of morphological and quantificational analysis,the specimens were harvested at 2,4 and 8 weeks after surgery.Results： 1） CPC/FG of 1：1 compared with the pure CPC,initial setting time extended,and final setting time is not statistically significant;Electron microscope found that fibrin glue between porous since solidified calcium phosphate crystals,and closely linked to it;2） A few immature vascular bubs could be found around the scaffolds two weeks affter surgery.The MVD of 1：1 group is higher than that of the CPC group（P0.05）.Four weeks after implantation,the peripheral blood vessel become mature.The microvessel density of the CPC/FG group was higher than that of the CPC group（P0.05）.Eight weeks after surgery,there was no more increasing of the number of microvessel,while the luments of microveessel had increased.There were no difference of microvessel density between four weeks and eight weeks in all the groups（P0.05）.Conclusion： Composite scaffold materials with proper coagulation time and better structure,the fibrin glue in composite materials and its dgradation have effect the formation of MVD in vivo,the vascularization of the composite has been enhanced,it can be used as cell carrier and defects of bone repair scaffold materials used in the clinical and experimental.
出处
《现代生物医学进展》
CAS
2013年第16期3024-3028,共5页
Progress in Modern Biomedicine
基金
国家自然科学基金青年科学基金项目(81000790)
北京市新星项目:2010年度北京市科技新星计划-崔赓(2010-77)
关键词
多孔自凝固磷酸钙
纤维蛋白胶
微血管密度
量化分析
凝固时间
Porous Calcium Phosphate Cements
Fibrin Glue
Microvessel density（MVD）
Vascularization
Setting time
