摘要
背景:目前国内外有关组织工程骨材料及构建方法的研究持续升温,而临床上如何修复大面积骨缺损仍然是研究的难点和重点。目的:对比观察同种异体骨支架材料、β-磷酸三钙材料以及碱性成纤维细胞生长因子慢病毒载体转染羊骨髓间充质干细胞的组织工程骨对羊髂骨极限骨缺损的修复效果。方法:体外诱导培养羊骨髓间充质干细胞至第3代时,构建碱性成纤维细胞生长因子慢病毒载体。将转染及未转染的骨髓间充质干细胞同种异体支架骨材料、单纯同种异体骨支架材料、β-磷酸三钙材料回植入羊髂骨极限缺损模型(15mm×10mm×10mm),修复后4,8,12周末进行影像学、组织学及扫描电镜观察。结果与结论:碱性成纤维细胞生长因子慢病毒转染羊骨髓间充质干细胞后的组织工程骨材料修复极限骨缺损优于未转染的细胞骨支架材料、单纯同种异体骨支架材料及β-磷酸三钙材料;未转染的细胞骨支架材料优于单纯同种异体骨支架材料与β-磷酸三钙材料组;单纯同种异体骨支架材料降解速率大于β-磷酸三钙材料。结果提示,以碱性成纤维细胞生长因子慢病毒载体转染羊骨髓间充质干细胞加载同种异体骨支架材料构建的组织工程骨符合骨修复的需求,既可以引导新骨形成,又不会妨碍骨组织的重建和塑型,具有良好的生物相容性。
BACKGROUND：Nowadays, the research regarding tissue engineering bone materials and construction methods continues to heat up, and how to repair large bone defects is stil difficult in the clinic. OBJECTIVE：To compare the effects of tissue engineering bones with al ogeneic scaffold material,β-tricalcium phosphate material and basic fibroblast growth factor lentiviral vector-transfected bone marrow mesenchymal stem cel s on the repairing of critical-sized iliac defects in sheep. METHODS：The sheep bone marrow mesenchymal stem cel s were in vitro induced and cultured to the third generation, and then the basic fibroblast growth factor lentiviral vector was constructed. The al ogeneic scaffold materials with transfected and untransfected bone marrow mesenchymal stem cel s, simple al ogeneic scaffold materials andβ-tricalcium phosphate materials were implanted into the sheep model of critical-sized iliac defects （15 mm×10 mm×10 mm）. Imaging, histology, and scanning electron microscopy observations were performed at 4, 8 and 12 weeks after repairing. RESULTS AND CONCLUSION：The repairing effect of al ogeneic scaffold materials with basic fibroblast growth factor lentiviral vector transfected bone marrow mesenchymal stem cel s on sheep critical-sized iliac defects was better than that of untransfected al ogeneic scaffold materials, simple al ogeneic scaffold materials andβ-tricalcium phosphate materials;the effect of untransfected al ogeneic scaffold materials was better than that of simple al ogeneic scaffold materials andβ-tricalcium phosphate materials;the degradation rate of simple al ogeneic scaffold materials was higher than that ofβ-tricalcium phosphate materials. The tissue engineering bone constructed using al ogeneic scaffold materials with basic fibroblast growth factor lentiviral vector-transfected bone marrow mesenchymal stem cel s can meet the requirement of bone repairing, and it can guide the new bone formation with good biocompatibility and cannot interfere with bone tissue reconstruction and modeling.
出处
《中国组织工程研究》
CAS
CSCD
2013年第16期2859-2868,共10页
Chinese Journal of Tissue Engineering Research
基金
国家自然科学基金地区基金项目(81060088)<三维打印构建组织工程化牙槽骨的实验研究>
新疆维吾尔自治区自然科学基金资助项目(2011211A073)~~
关键词
生物材料
组织工程骨材料
极限骨缺损
碱性成纤维细胞生长因子
慢病毒载体
骨髓间充质干细胞
组织工程骨
骨支架材料
β-磷酸三钙材料
国家自然科学基金
biomaterials tissue-engineered bone materials critical-sized defect basic fibroblast growth factor lentiviral vector bone marrow mesenchymal stem cells tissue engineering bone bone scaffold materials β-tricalcium phosphate materials National Natural Science Foundation of China
