摘要
目的 调查山东省的莱姆病自然疫源地。方法 于 1992～ 1999年 ,在山东省的山林地区设点 ,用间接免疫荧光抗体试验进行人群莱姆病感染调查 ,结合流行病学史、临床表现和实验室检查结果进行莱姆病病例诊断 ;对主要媒介蜱进行调查 ,用布旗法采集蜱类 ,分类鉴定 ,直接免疫荧光抗体法进行中肠带螺旋体率的调查 ,用BSK培养基分离病原 ;宿主动物调查 ,用鼠笼和鼠夹捕获鼠类 ,分类 ,解剖 ,取肾脏、膀胱等 ,用BSK培养基分离病原。结果 山东省的山林地区人群中存在莱姆病的感染 ,平均感染率为 6 .2 5 % ,经个案流行病学调查 ,结合临床症状和血清学检查结果确诊典型病例19例 ;该地区的蜱类以长角血蜱为优势种 ,该蜱中肠携带莱姆病螺旋体率为 12 .0 % ,从 86组长角血蜱培养物中获得 2株莱姆病螺旋体 ;肾脏带菌率检查 46只鼠类 ,发现 6只鼠的肾脏内带有典型的莱姆病螺旋体 ,带菌率为 13.0 4%。
Objective To investigate the endemic area of Lyme disease in Shandong province. Methods An investigation on endemic Lyme disease was conducted by means of serological and etiological methods in Shandong province from 1992 to 1999. Results Of 1919 forestry residents, 120 residents had a significant antibody titer against Borolo burgdorferi strain B 31 . The prevalance rate of Lyme disease was 6.25 %. Haemaphysalis longicornis was a prevailing species of Ixodidae in the region. Spirochetes were observed in the midguts of 50 H. longicornis in this area by direct fluorescence antibody method. The overall positive rate was 12.0 % (6/50). Two strains (TSH 1, TSH 3) of spirochetes were isolated from H. longicornis. Both TSH 1 and TSH 3 were positively responded to McAb H 5332 and H 9724 , but negatively to H 6831 . Spirochetes were observed in the kidneys of 46 Rattus in this area by direct fluorescence antibody method with a positive rate of 13.26 % (6/46). Conclusion It was the first time that the mountainous areas in Shandong province was confirmed endemic areas of Lyme disease.
出处
《中华流行病学杂志》
CAS
CSCD
北大核心
2000年第4期292-294,共3页
Chinese Journal of Epidemiology
关键词
莱姆病
莱姆病螺旋体
自然疫源地
长角血蜱
Lyme disease
Brrelia burgdorferi
Endemic area
Haemaphysalis longicornis
