摘要
应用AFLP方法对福建沿海葡萄牙牡蛎自然苗群体(福清、莆田、石狮、厦门)和人工苗群体(宁德、连江、石狮、诏安)进行了遗传多样性分析。采用4对选择性引物组合对8个养殖群体239个个体进行扩增,共得到331个位点,多态位点233个。8个养殖群体的多态位点比例、Nei’s基因多样性指数和Shannon遗传多样性指数分别为57.10%～69.54%,0.196 7～0.249 2,0.290 2～0.362 6。其中4个自然苗群体的多态位点比例、Nei’s基因多样性指数和Shannon遗传多样性指数均高于4个人工苗群体。遗传分化系数Gst及AMOVA分析表明,8个养殖群体的遗传变异主要来源于群体内个体间。UPGMA聚类图及PCA聚类图分析表明,人工苗群体和自然苗群体之间及人工苗群体内都存在一定的遗传分化,而自然苗群体内所有个体基本是随机交叉聚类,没有形成明显的类群分支。以上结果表明,自然苗群体的遗传多样性高于人工苗群体,群体间存在一定的遗传分化。
Genetic diversity of cultivated Crassostrea angulata in coastal sea of Fujian was analyzed by amplified fragment length polymorphism （AFLP）. A total of 331 loci reproducible bands amplified with four AFLP primer combinations were assessed from 239 individuals in the eight domestic populations including four natural seeding cultivated populations and four artificial breeding populations, of which 233 （70.39%） were polymorphic. The proportion of polymorphie loci, the Nei＇s gene diversity and Shannon＇ s Information Index of 8 cultivated populations were 57.10% - 69.54%, 0. 196 7 - 0. 249 2, 0.290 2 - 0. 362 6, respectively. Gst value and the AMOVA analysis indicated that the genetic variation mainly came from different individuals within the population. Cluster analysis based on UPGMA and PCA showed that genetic variation occurred within groups for each population, and there was no distinct boundary within the four natural seeding populations. Results showed that the genetic diversity of natural seeding cultivated populations had higher genetic diversity compared with artificial breeding cultivated populations, and a certain degree of genetic differentiation among populations was observed.
出处
《上海海洋大学学报》
CAS
CSCD
北大核心
2013年第3期328-333,共6页
Journal of Shanghai Ocean University
基金
现代农业产业技术体系建设专项(nycytx-47)
国家科技基础条件平台(水产种质资源平台)
福建省科技计划项目基本科研专项(2009R10011-6)
