摘要
采用正交试验确定大肠杆菌BL21(DE3)产β-环糊精转移酶的发酵条件。结果表明,生产β-环状糊精转移酶的最适条件为:接种量1%,100mL三角瓶装液量15mL,初始pH值为7.0,Ca2+浓度为1.25mmol/L,Mg2+浓度为2.50mmol/L,温度控制在37℃,转速控制为150r/min。当OD600达到1.4时,加入终浓度5.0g/L的α-乳糖,转速提高至170r/min,37℃诱导12h后过滤,β-CGTase酶活最高可达16.3μ/mL。
The culture condition for producing β-cyclodextrin glucanotransferase by E.coli BL21 （DE3） was primarily established by orthogonal experimental design. The results showed that the optimal fermentation conditions were as follows： the inoculum size was 1%; The load of 100ml-flask was 15ml; initial pH value was 7.0; the concentration of Ca^2＋ was 1.25mmol/L, the concentration of Mg^2＋ was 2.50mmol/L, incubation temperature was 37~C and the speed was 150r/rain. When the value of OD600 reached 1.4, u-lactose was added so as to the final concentration to 5.0g/L, the speed was increased to 170r/min, and induced 12h at 37℃ then filtered, the β-CGTase activity reached the maximum value as 16.3μ/ml.
出处
《中国酿造》
CAS
2013年第4期52-57,共6页
China Brewing
基金
科技部农业科技成果转化资金项(2010GB2F300436)
国家自然科学基金委员会资助项目(31160229)
