摘要
建立了高效液相色谱法同时测定精油中5种禁用性激素炔诺孕酮、醋酸甲地孕酮、醋酸氯地孕酮、醋酸甲羟孕酮、丙酸睾丸酮含量的方法。精油样品采用90%甲醇-水(体积比)溶液提取,正己烷除脂后,以Kromasil100-5C18柱(250mm×4.6mm,5μm)分离,乙腈-水(65∶35,体积比)为流动相,二极管阵列检测器以242nm和290nm为检测波长进行定性和定量分析。该方法对精油中5种性激素分离效果良好,在100～10000μg/L范围内呈良好线性关系,相关系数大于0.9990;五种性激素的检出限为0.550～5.040mg/kg,定量限为0.662～5.260mg/kg;除丙酸睾丸酮外,实际样品中加标回收率为88%～120%,相对标准偏差<4%。该方法操作简便,定值准确,能够满足精油中性激素的检测要求。
A comprehensive analytical method based on high performance liquid chromatography （HPLC） has been developed for the determination of 5 sexual hormones （norgestrel, megestrol-17-acetate, chloromadinone 17 acetate, medroxyprogester- one 17 acetate, testosterone propionate） in essential oil. The cosmetic samples were extracted by 90% methanol water. The target compounds were separated on an Kromasil 100-5C18 column（250 mm×4.6 mm,5 μm） using acetonitrile-water（65/35） as mobile phases. The detective wavelength was 242 and 290 nm. The linear plots were obtained between 100 and 10 000 μg/L with good correlation coefficients larger than 0.9990. The limits of detection （LODs） for this method were in the range of 0.550-5.040 mg/kg, the qualification of detection （QOD） were between 0.662 5.260 mg/kg. The recoveries were in the range of 90%-120% except testosterone propionate. The relative standard deviations （RSD） were 〈4%. The results indicated that the method is simple, rapid, accurate and suitable for the determination of 5 sexual hormones in essential oil.
出处
《香料香精化妆品》
CAS
2013年第2期48-51,共4页
Flavour Fragrance Cosmetics
基金
国家质量监督检验检疫总局化妆品中激素类药物的检测方法研究资助(201110245-02)
关键词
高效液相色谱法
性激素
精油
high performance liquid chromatography （HPLC） sexual hormones essential oil
