摘要
本研究旨在了解猪源新型甲型H1N1流感病毒山东分离株的遗传进化特点。对山东地区出现的疑似H1N1流感病死猪进行病料采样,然后进行病毒的分离鉴定,并对分离病毒株(A/swine/Shandong/07/2011)的HA、NA、PB2、PB1、PA、NP、NS和M基因进行遗传进化分析。结果显示,该株病毒8个片段的核酸序列与A/H1N1(2009)对应序列的相似性都大于99%,并且该毒株HA蛋白的裂解位点和优先识别唾液酸α-2,6受体的位点与A/H1N1(2009)也高度一致,分别为PSIQSR↓GLFGAI和190D、225D。但是,与A/H1N1(2009)毒株的HA蛋白相比,受体结合位点处出现了重要的突变(Q226R)。该研究结果为进一步研究猪源新型甲型H1N1流感病毒的分子进化提供了重要信息。
The study was carried out to examine the genetic characteristics of a novel swine-origin influenza A （H1N1） virus isolated from Shandong province. Samples from suspected influenza-in- fected pigs were collected for viral isolation and identification, and then genetic evolution of HA, NA,PB2,PB1,PA, NP, NS and M of the isolate （A/swine/Shandong/07/2011） was analyzed and compared with the related influenza viruses. The results showed that sequences of 8 frag- ments of the isolated virus revealed 〉99% nucleotide identity with A/HIN1 （2009） prototype strain, and its HA cleavage and receptor-binding sites were PSIQSR $ GLFGAI and 190D, 225 D, respectively, which were identifical with A/H1NI（2009） . However, compared with HA pro- tein of A/HINl（2009）, mutation Q226R occurred in receptor binding site of the isolate. In con- clusion, the results of this study provided significant information for further research on molecu- lar evolution of novel swine-origin influenza A （HIN1） virus.
出处
《畜牧兽医学报》
CAS
CSCD
北大核心
2013年第4期583-591,共9页
ACTA VETERINARIA ET ZOOTECHNICA SINICA
基金
国家环境保护环境微生物利用与安全控制重点实验2011年开放基金(MARC2011D061)
国家科技支撑计划子课题(2010BAD04B000)
关键词
山东地区
猪源新型甲型H1N1流感病毒
分离鉴定
相似性
基因序列分析
Shandong region
novel swine-origin influenza A （HIN1） virus
isolation and identifi-cation
nucleotide identity~ phylogenetic analysis
