摘要
采用平板稀释分离培养法和BIOLOG-ECO微平板法对三峡库区小江流域消落区的土壤微生物数量和代谢多样性进行了研究。结果表明:可培养微生物数量随沿程变化较为明显,同时受到时间变化影响;5个采样点微生物利用单一碳源的能力随时间的变化逐渐增大;大多数采样点微生物群落的丰富度和优势度不随时间变化且采样点之间的丰富度和优势度差异不明显,随时间的变化个别采样点微生物群落的均匀度受到了影响且不同采样点之间差异较明显;主成分分析结果显示5个采样点土壤微生物在利用碳源方式上有差异。
Large water-level-fluctuation zone was formed after the impoundment of the Three Gorges Reservoir. The microbial diversity of this area could be greatly influenced by the water level fluctuation. The enumeration and BIOLOG-ECO methods were used to assess the microbial diversity in the water-level-fluctuation zone. Results showed that the quantity of cultured microorganisms was highly dependent on the position. The quantity of cultured microorganisms changed over time. The data of BIOLOG demonstrated clearly that the diversity index of most soil samples did not changed significantly. The soil microbes from different soil samples utilized different carbon sources, and their ability to utilize certain carbon sources increased with time.
出处
《华中农业大学学报》
CAS
CSCD
北大核心
2013年第3期15-20,共6页
Journal of Huazhong Agricultural University
基金
国家水体污染控制与治理科技重大专项(2009ZX07104-003-04)
关键词
三峡库区
消落区
微生物多样性
平板稀释分离培养法
BIOLOG-ECO法
Three Gorges Reservoir
water-level-fluctuation zone
microbial diversity
dilution- plate isolation and culture method
BIOLOG-ECO method
