摘要
本试验用5%二甲基甲酰胺(DMF)替代甘油作为冷冻保护剂,研究不同浓度(0、0.02、0.04、0.06、0.08 mg/mL)L-肉碱对猪精液冻后常规指标(精子活率、线粒体活性、顶体完整性、质膜完整性)、过氧化氢酶(CAT)和总抗氧化酶(T-AOC)活性以及丙二醛(MDA)含量的影响。结果表明:添加0.04和0.06 mg/mL的L-肉碱可有效改善猪精液冷冻后效果。0.04 mg/mL组可显著提高精子冻后活率和线粒体活性(P<0.05);0.06 mg/mL组可显著提高顶体完整性和质膜完整性(P<0.05)。添加0.06 mg/mL L-肉碱可显著提高冷冻后精子内T-AOC酶活性并且抑制MDA的产生(P<0.05),但CAT活性与0.04 mg/mL组差异不显著。在冷冻稀释液中添加0.06 mg/mL L-肉碱可以提高猪精液冷冻保存效果。
In this experiment, glycerol was replaced with 5% dimethyl formamide （DMF） completely and the L-carnitine of different con- centrations （0, 0. 02, 0.04, 0. 06, 0. 08 mg/mL） were added in the ordinary dilution, then the routine indicaters （ sperm viability, mito- chondrial activity, acrosome integrity and membrane integrity） , the activity of CAT （Catalase） and T-AOC （total antioxidant capacity） and the content of MDA （Malondialdehyde） of frozen-thawed boar sperm were analyzed. The result showed that the cryoprotective effect were im- proved with addition of 0. 04 and 0. 06 mg/mL L-carnitine. When the L-carnitine concentration was 0. 04 mg/mL, the sperm viability and mitochondrial activity were significantly higher than that of control group and other groups （P〈0. 05 ）. When the L-carnitine concentration was 0. 06 mg/mL, the acrosome integrity and membrane integrity were significantly higher than that of control group and other groups （ P〈 0. 05）. The activity of T-AOC was increased and the content of MDA was decreased significantly with addition of 0. 06 mg/mL L-carnitine, but there have no significant differences on the activity of CAT between 0. 04 and 0.06 mg/mL groups. These results indicated that L-carni- tine could improve boar semen quality ＇after cryoprotection and the appropriate addition of L-carnitine in ordinary dilution was 0. 06 mg/mL.
出处
《畜牧与兽医》
北大核心
2013年第4期14-17,共4页
Animal Husbandry & Veterinary Medicine
基金
科技部支撑项目(2011BAD19B04)
