摘要
目的 探讨CD34和血管内皮生长因子 (VEGF)在人肝癌组织中的表达及微血管密度(MVD)的病理意义。方法 对 30例人肝细胞肝癌 (HCC)及相应癌旁组织 ,10例肝硬化 ,5例轻度慢性肝炎和 4例正常肝组织 ,进行了CD34、VEGF免疫组织化学SP法检测 ,对CD34阳性血管进行MVD计数 ,对VEGF进行半定量计数 ,并结合肝癌的病理特征进行分析。结果 HCC组织中CD34呈广泛 ,窦隙状阳性表达 ,而在正常及慢性肝炎组织中肝窦呈阴性 ,在纤维间隔附近的肝窦壁可见少量散在的CD34弱阳性表达 ,MVD与HCC的肿瘤大小及是否发生门静脉癌栓密切相关 ,瘤块直径 <2cm者的MVD显著低于 >2cm者 ,VEGF阳性率为 83.3 % (2 5 /30 ) ,而在 5例慢性肝炎患者中为阴性表达 ,并与肿瘤发生门静脉癌栓关系密切 (P <0 .0 5 )。瘤旁肝组织亦显示较明显的VEGF阳性 (93 .3% ,2 8/30 )。结论 CD34和VEGF的阳性表达为HCC与非癌性肝组织的鉴别诊断提供了较为可靠的形态学标记。瘤组织可通过自分泌和旁分泌的VEGF促进肿瘤血管形成。
Objective To study clinicopathologic significance of microvessel density (MVD) and the expression of vascular endothelial growth factor (VEGF) in human hepato-cellular carcinoma(HCC). Methods 30 HCC cases with neighboring noncancerous tissues, 10 liver cirrhosis cases, 5 mild chronic hepatitis cases and 4 normal liver tissue samples were stained with CD34, VEGF by immunohistochemistry method. Quantitation of sinusoid-like vessels and semi-quantitation of VEGF expression were performed and analyzed in conjunction with the clinico-pathological features of HCC. Results All HCC tissues showed diffuse CD34 expression and its site of distribution was somewhat corresponding to the original structure of sinusoid-like pattern, while CD34 was negative in the “sinusoid” regions of normal and chronic liver disease. Both MVD and VEGF were correlated to portal vein invasion of HCC, MVD was also correlated to the tumor size. The MVD of tumor less than 2 cm in diameter was significantly lower than that of tumors with a diameter of 2～5 cm. The positive rate of VEGF in HCC was 83.3%. The expression of VEGF was also remarkable in the neighboring noncancerous tissues in which inflammatory cell infiltration was apparent. Conclusions MVD and expression of VEGF were remarkably increased in HCC. Both were correlated with portal vein invasion. VEGF derived from tumor cells and noncancerous tissues may be associated with the angiogenesis of HCC.
出处
《中华病理学杂志》
CSCD
北大核心
2000年第4期248-251,共4页
Chinese Journal of Pathology
关键词
肝肿瘤
微血管密度
血管内皮生长因子
CD34
Liver Neoplasms
Neovascularization
pathologic
Endothelial growth factors
Antigens CD34
Diagnosis
differential
