摘要
短程硝化是污水脱氮工艺中的重要环节,系统中的菌群结构决定了其处理效果.为探讨短程硝化系统中的微生物对不同污水的适应性,利用细菌16S rDNA克隆文库、磷脂脂肪酸(PLFA)和定量PCR分析方法对高氨氮废水和城市生活污水短程硝化系统中活性污泥的细菌群落结构、总体微生物的多样性以及功能微生物进行了比较.克隆文库结果表明两个系统中细菌群落结构明显不同,城市生活污水中细菌种类更丰富,但两个系统的优势菌群都属于变形菌门(Proteobacteria)和拟杆菌门(Bacteroidete).磷脂脂肪酸分析结果显示高氨氮废水短程硝化系统中微生物多样性指数和均匀度指数明显低于城市生活污水.定量PCR结果表明,高氨氮废水短程硝化系统中氨氧化菌(AOB)与亚硝酸盐氧化菌(NOB)的数量都多于城市生活污水短程硝化系统;高氨氮废水短程硝化系统中AOB比NOB高3个数量级,而城市生活污水短程硝化系统中AOB比NOB高2个数量级.
Nitritation is an important part of the biological nitrogen removal process,and the performance of the process was determined by the microbial community structure.To explore the microbial adaptability to different sewage,the microbial diversity and the amount of bacteria were investigated in a high ammonia wastewater treatment process and a sewage treatment process using the clone library of bacterial 16S rDNA,the phospholipid fatty acid method（PLFA） and the quantitative PCR.The clone library results showed that there was a significantly difference in bacterial community structure of these two processes,although the dominant bacteria belong to the Proteobacteria and Bacteroidete,there were more clusters in the sewage treatment process.The PLFAs results showed that the microbial diversity index and the evenness index of the high ammonium wastewater treatment process were significantly low.The quantitative PCR results showed that amounts of ammonia oxidizing bacteria（AOB） and nitrite-oxidizing bacteria（NOB） in the high ammonium wastewater treatment process were higher than these in sewage treatment process.The copy number of AOB was higher than the copy number of NOB in the high ammonia wastewater treatment process by three orders magnitude.The copy number of AOB was higher than the copy number of NOB in sewage treatment process by two orders of magnitude.
出处
《环境科学》
EI
CAS
CSCD
北大核心
2013年第4期1448-1456,共9页
Environmental Science
基金
中国科学院知识创新工程项目(KZZD-EW-09-3
KZCX2-YW-JC407-3
KSCX2-YW-G-054-2)
关键词
短程硝化
生物脱氮
16S
rDNA克隆文库
PLFA
定量PCR
partial nitritation
biological nitrogen removal
16S rDNA clone library
phospholipid fatty acid（PLFA）
quantitative PCR
