摘要
[目的]选出最适宜诱导甜瓜黄醉仙子叶愈伤组织和不定芽的培养基,以及适宜其不定芽伸长和生根的培养基。[方法]以甜瓜黄醉仙子叶为外植体,研究添加不同浓度BA和IAA激素组合的MS培养基对其愈伤组织和不定芽诱导及不定芽伸长的影响,以及添加不同浓度IBA激素组合的MS培养基对其生根的影响。[结果]最适宜诱导甜瓜黄醉仙子叶愈伤组织培养基是MS+0.5 mg/L BA+2.0mg/L IAA和MS+1.0 mg/L BA+2.0 mg/L IAA,最适宜其子叶不定芽诱导的培养基是MS+0.5 mg/L BA+0.5 mg/L IAA,不定芽伸长的最适宜培养基是MS+1.0 mg/L IAA,生根的适宜培养基是1/2MS+1.0 mg/L IBA。[结论]初步选出一套完整适宜甜瓜黄醉仙子叶再生体系建立的培养基。
[Objective] To choose the most suitable induced melon Huangzuixian leaves callus and adventitious bud medium,and suitable for the adventitious bud elongation and rooting medium.[Method] With melon Huangzuixian seed as the materials,cotyledon as explants,adding different concentration of BA and IAA hormone combination of the MS medium on the callus and adventitious bud induction,adventitious bud elongation and the effects of adding different concentration IBA hormone combination of the MS culture medium on the rooting effect were studied.[Result] The most suitable induced melon Huangzuixian leaves callus culture medium is MS ＋ 0.5 mg/L BA ＋ 2.0 mg/L IAA and MS ＋ 1.0 mg/L BA ＋ 2.0 mg/L IAA,the most suitable for its cotyledon adventitious bud induction medium is MS ＋ 0.5 mg/L BA ＋ 0.5 mg/L IAA,adventitious bud elongation of the most suitable medium is MS ＋ 1.0 mg/L IAA rooted appropriate medium is 1/2 MS ＋ 1.0 mg/L IBA.[Conclusion] A culture medium which is suitable for melon Huangzuixian leaves regeneration system was selected.
出处
《安徽农业科学》
CAS
2013年第5期1908-1910,共3页
Journal of Anhui Agricultural Sciences
基金
新疆维吾尔自治区公益性科研院所基本科研业务经费项目(KY2012080)
关键词
甜瓜
黄醉仙
再生体系
子叶
Melon
Huangzuixian
Regeneration system
Cotyledon
