摘要
目的:建立HPLC法测定盐酸曲马葡萄糖注射液中盐酸曲马多的含量。方法:色谱柱:Bondysil-C_(18)(250 mm×4.6 mm,5μm),流动相:醋酸-冰醋酸钠缓冲液(pH4.5)-甲醇(65:35),流速:1.0 ml·min^(-1),柱温:35℃,检测波长:271 nm,进样量:20μl。结果:盐酸曲马多质量浓度线性范围为10～140μg·ml^(-1)(r=0.999 0),平均回收率为99.9%(RSD=0.80%,n=9)。结论:本方法简便、灵敏、准确。
Objective：To establish an HPLC method for the determination of tramadol hydrochloride in tramadol hydrochloride glucose injections.Method：The analysis was performed on a Bondysil-C_（18）（250 mm×4.6 mm,5μm） column.The mobile phase was acetic acid-sodium glacial acetic acid buffer（pH 4.5） and methanol（65：35）.The flow rate was 1.0 ml·min^（-1）,the column temperature was 30℃,the detection wavelength was 271 nm,and the injection volume was 20μl.Result：The linear range of tramadol hydrochloride was 10-140μg·ml^（-1）（r= 0.999 0）,and the average recovery was 99.9%with RSD of 0.80%（n= 9）.Conclusion：The method is simple,sensitive and accurate.
出处
《中国药师》
CAS
2013年第3期393-394,共2页
China Pharmacist
