摘要
[目的]白子菜在临床和民间广泛用于治疗高血压病、高脂血症及糖尿病,2010年卫生部将其列为新资源食品。本文采用高效液相色谱法（HPLC）建立白子菜中的主要黄酮类成分（芦丁）的含量测定方法。[方法]石油醚（30～60℃）前处理,取样量1.000 g,60%乙醇40 mL,超生提取15 min。色谱柱：Xterra@RP 185 um;流动相：甲醇-1%冰醋酸（40∶60）;检测波长：360 nm。[结果]通过线性关系考察,表明芦丁质量浓度在0.011 2-0.067 2 mg/mL范围内线性关系良好,r=0.999 1。精密度、重复性、重现性、稳定性及加样回收率结果均能满足分析要求。[结论]此方法简便,准确,可为测定白子菜中黄酮类成分（芦丁）的含量,提供一定的参考。
[Objective] To establish assay method about the main ingredients of rutin in Gynura divaricata with HPLC. [Methods] Pre-treatment was performed by petroleum ether （30-60℃）. Mobile phase was “methanol-1% glacial acetic acid （40：60）”. The detection wavelength was 360 nm. Sample volume was 40 mL 1.000g 60 % ethanol extract. Ultra extract was processed for 15 min. Chromato-graphic column was Xterra @RP 185 urn. [Results] According to the linear relationship, it was showed that linear relation of rutin mass concentration was good in 0.011 2-0.067 2 mg/mL, r=0.999 1. All accuracy, reproducibility, stability and recovery could meet the analy-sis demand. [Conclusion] This method is simple and accurate, and it can provide some certain references for determination of rutin con-tent in Gynura divaricata.
出处
《天津中医药》
CAS
2013年第2期112-114,共3页
Tianjin Journal of Traditional Chinese Medicine
