摘要
目的了解儿童EB病毒(EBV)感染情况,并分析其相关疾病谱,从而为EBV感染及相关疾病的防治提供科学的理论依据。方法采用real-time PCR法检测2010年8月至2011年7月收治的761例(年龄22 d至14岁)疑似EBV感染儿童血浆中EBV-DNA载量,并对EBV-DNA检查结果及相关疾病进行统计学分析。结果 761例血浆标本中EBV-DNA阳性标本109例,阳性率为14.3%;不同年龄组EBV-DNA阳性检出率差异有统计学意义(P<0.05),其中婴儿组(<1岁)的阳性检出率最低(P<0.05);不同季节间阳性检出率差异有统计学意义(P<0.05),其中夏季阳性检出率高于冬季(P<0.05)。109例阳性标本的EBV-DNA载量范围为2.13～6.69,中位数为3.72。对62例EBV-DNA阳性住院患儿最终临床诊断分析得出,呼吸系统疾病占39%,主要为急性支气管炎、急性上呼吸道感染及急性支气管肺炎。结论不同年龄组及不同季节间EBV-DNA阳性检出率不同;儿童EBV感染相关疾病以呼吸系统疾病为主;Real-time PCR法检测血浆EBV-DNA有助于临床上EBV感染的早期诊断。
Objective To study the status of EB virus （EBV） infection and the spectrum of EBV infection-related diseases. Methods A total of 761 plasma samples with suspected EBV infection were collected from 761 children （ aged from 22 days to 14 years） admitted between August 2010 and July 2011. E BV-DNA of 761 plasma samples was detected by real-time PCR. The epidemiological characteristics and final clinical diagnosis were analyzed based on the clinical data of these EBV-positive hospitalized patients. Results A total of 109 cases with EBV infection were detected by real-time PCR, with a positive rate of 14.3%. There were significant differences in the positive rate of EBV-DNA among different age groups and between seasons （P 〈0.05）. The positive rate of EBV-DNA in the baby group （ 〈 1 year old） was lowest （P 〈 0.05 ）, and the positive rate of EBV-DNA in summer was higher than in winter （ P 〈 0.05 ）. The range of plasma EBV-DNA level in children with EBV-DNA positivity was 2.13 to 6.69 （ median 3.72）. Based on the final diagnosis of 62 EBV-positive hospitalized children, the most common disease was respiratory system infection （39%）, such as acute bronchitis, acute upper respiratory infection and bronchopneumonia. Conclusions The EBV-DNA positive rate is different among different age groups and between seasons. Respiratory system infection is a leading disease in hospitalized children who are EBV-DNA positive. Real-time PCR assay is useful for rapid and reliable clinical diagnosis of EBV in children.
出处
《中国当代儿科杂志》
CAS
CSCD
北大核心
2013年第3期183-186,共4页
Chinese Journal of Contemporary Pediatrics
关键词
EB病毒
感染
呼吸系统疾病
儿童
Epstein-Barr virus
Infection
Respiratory system disease
Child
