摘要
目的从基因水平探讨湖北地区汉族人食管癌 HEN-DQB1等位基因的遗传易感性.方法运用序列特异性引物聚合酶链反应技术,检测无亲缘关系湖北汉族健康人136例、食管癌组42例患者的 HLA-DQB1等位基因.SAS system 统计软件数据处理.结果湖北汉族人食管癌患者与正常人比较,HEN-DQB1*0301基因频率显著增高(0.2976 vs 0.1875),P=0.046,OR=1.835,病因分数=0.1354);两组间 HLA-DQB1其余各等位基因分布频率的比较,HLA-DQB1*0201(0.0833 vs 0.1016),*0301(0.2976 vs 0.1875),*0302(0.0595 vs 0859),*0303(0.2381 vs 0.1875),*0304(0.0000 vs 0.0039),*0401(0.0714 vs 0.0469),*0402(0.0119 vs 0.0156),*0501(0.0357 vs 0.0703),*0502(0.0595 vs 0.0664),*0503(0.0119 vs 0.0195),*0504(0.0000 vs 0.0039),*0601(0.0595 vs 0.0781),*0602(0.0476 vs 0.0742),*0603(0.0000 vs 0.0078),*0604(0.0238 vs 0.0508),差异均无显著性.结论 HLA-DQB1*0301等位基因与湖北汉族人食管癌正关联,为其易感基因.
AIM To probe into the genetic susceptibility of HLA- DQB1 alleles to esophageal cancer in Hubei Chinese Hans. METHODS HLA-DQB1 gene polymorphisms were typed by sequence specific primer based polymerase chain reaction,in 42 patients with esophageal neoplasm and 136 normal control subjects.SAS softword (6.12 for window) was used in satistics. RESULTS Gene frequency (GF) of HLA-DQB1*0301 allele was significantly higher in esophageal neoplasm patients than those in normal controls,0.2976 vs 0.1875, P=0.046,the odds ratio 1.835,etiologic fraction 0.1354. There was no association between the patients with the controls in the rested HLA-DQB1 alleles,i.e.GF:HLA- DQB1*0201 (0.0833 vs 0.1016),*0301 (0.2976 vs 0.1875) *0302 (0.0595 vs 0.0859) *0303 (0.2381 vs 0.1875) *0304 (0.0000 vs 0.0039) *0401 (0.0714 vs 0.0469) *0402 (0.0119 vs 0.0156) *0501 (0.0357 vs 0.0703) *0502 (0.0595 vs 0.0664) *0503 (0.0119 vs 0,0195) *0504 (0.0000 vs 0.0039) *0601 (0.0595 vs 0.0781) *0602 (0.0476 vs 0.0742) *0603 (0.0000 vs 0.0078) *0604 (0.0238 vs 0.0508), CONCLUSION HLA-DQB1 *0301 allele is positively susceptibilitive to the esophageal carcinoma patients of Hubei Han Chinese.Individuals carrying HLA-DQB1*0301 may be susceptible to esophageal carcinoma.
出处
《世界华人消化杂志》
CAS
2000年第9期965-968,共4页
World Chinese Journal of Digestology
关键词
食管癌
HLA-DQB1
基因多态性
遗传易感性
esophageal neoplasm
aberrant cell
HLA-DQB1
sequence specific primer based polymerase chain reaction
gene amplification
genetic susceptibility
antigens,neoplasm
cell adhesion molecules
