摘要
目的研究金叶过路黄在NaCl胁迫下,反映抵抗逆境的各项生理指标的变化及胁迫对其叶片挥发性成分的影响。方法采用不同浓度(150、200、250、300 mmol·L-1)NaCl营养液对金叶过路黄幼苗进行不同强度的盐胁迫处理,测定叶片可溶性糖、脯氨酸和SOD等8项生理指标的变化,并用顶空固相微萃取与气质联用技术对金叶过路黄叶中挥发性成分进行分析。结果随着盐胁迫程度的加深,渗透调节物质中可溶性蛋白质的含量逐渐下降,可溶性糖的含量逐渐升高,脯氨酸的含量在250 mmol·L-1盐浓度胁迫时达到最大值,叶绿素a和b含量呈先降后升的趋势,在300 mmol·L-1含量有所升高。MDA含量和SOD总活性随着胁迫浓度的增大而增大,4个不同浓度盐胁迫组的POD活力显著低于对照组,CAT活力在轻度盐胁迫时有所上升,增大胁迫浓度,活力迅速下降。金叶过路黄叶中的挥发性成分在盐胁迫后有所变化。结论金叶过路黄在盐胁迫时主动积累渗透调节物质,增强抗氧化酶清除活性氧自由基的能力,具有较好的抗盐性。但盐胁迫影响其挥发性成分,可能进而影响其药用活性。
OBJECTIVE To study the changes in physiological indices reflecting resistance to environmental stress and volatile constituents of Lysimachia nummularia Aurea. METHODS Different concentrations （ 150,200,250 and 300 mmol ·L^-1 ） of NaC1 solution was used to treat young Lysimachia nummularia Aurea to investigate the effect of saline stress on the seedling growth. The changes of physiological indices including soluble sugars, proline, SOD and so on were assayed. Volatile constituents were analyzed by head space solid-phase microextration （HS-SPME） coupled with gas chromatogTaphy-mass spectrometry （GC/MS）. RESULTS With increasing stress intensity, the contents of soluble proteins declined, those of soluble sugars increased gradually, the content of proline reached the maximum value when saline stress intensity was 250 mmol ·L^-1, and the contents of chlorophyll a and b decreased firstly and rose later above 250 mmol ·L^-1 MDA content and SOD activity rose gradually under saline stress with increasing stress intensity. POD activity was significantly lower under the saline stress than the control group. CAT activity rose firstly and rapidly declined later. The volatile constituents of L. nummularia fluctuated ＂,after saline stress. CONCLUSION L. nummularia has relatively higher saline resistance by means of active accumulating osmoregulation substances such as soluble sugars, soluble proteins and enhancing capability of antioxidase.
出处
《中国药学杂志》
CAS
CSCD
北大核心
2013年第6期423-427,共5页
Chinese Pharmaceutical Journal
基金
河南省科技厅重点攻关项目(102102310019
12210231070)
河南省科技厅基础前沿计划(1102300410075)
河南省青年骨干教师计划([2012]704)
关键词
金叶过路黄
盐胁迫
生理指标
挥发性成分
Lysimachia nummularia Aurea
NaC1 stress
physiological indices
volatile constituent
