摘要
目的 :研究缺血再灌注损伤后肠粘膜上皮细胞凋亡与 ICE和 bcl 2基因 m RNA表达的关系 ,并探讨药用大黄的肠道屏障保护作用机制。方法 :制备小鼠肠系膜上动脉 (SMA)缺血再灌注损伤的实验模型 ,琼脂糖凝胶电泳法检测肠上皮细胞凋亡 ,逆转录定量多聚酶链式反应 (RT Q PCR)检测肠上皮细胞中 ICE和bcl 2 m RNA的表达。结果 :大黄治疗组小鼠肠上皮细胞致伤后各时间点 DNA梯状电泳条带强度显著弱于对照组小鼠。 bcl 2基因在 2组小鼠肠上皮细胞各时间点均呈低水平表达 ;而大黄治疗组 ICE基因的表达与对照组相比均有显著性下降 (P均 <0 .0 5 )。结论 :缺血再灌注损伤过程中 ,ICE基因的持续高水平表达可能是导致肠上皮细胞发生异常凋亡的重要原因 ,致伤后立即应用大黄煎剂可以下调肠上皮细胞 ICE基因的 m RNA表达 ,减轻肠上皮细胞的凋亡程度。
Objective:To investigate the relationship between the apoptosis characteristics of intestinal epithelial cells and the levels of gene transcription of ICE and bcl2 after intestinal ischemiareperfusion injury,and to clarify the protective role of Rheum officinale Baill to gut barrier function and its possible mechanisms.Methods:The animal model of supermesenteric artery (SMA) ischemiareperfusion in mouse was established.Apoptosis of intestinal epithelial cells was determined by agarose gel electrophoresis and the mRNA levels of ICE and bcl2 were assayed using reverse transcription quantitative polymerase chain reaction(RTQPCR).Results:The intensity of DNA ladder of intestinal epithelial cells in Rheum officinale Baill treated group was significantly lower than that in the control group.While the bcl2 mRNA transcription decreased in both groups after injury,the ICE mRNA transcription in Rheum officinale Baill treated group was significantly downregulated as compared with that in the control group (all P <0 05).Conclusions:The persistent high transcription level of ICE gene may be an important causative factor of abnormally apoptosis in intestinal epithelial cells during ischemiareperfusion injury.Application of Rheum officinale Baill immediately after injury can lessen the degree of such apoptosis through the downregulation of the ICE gene transcription.
出处
《中国危重病急救医学》
CAS
CSCD
2000年第7期387-389,共3页
Chinese Critical Care Medicine
基金
国家自然科学基金!资助项目 ( 3 9670 70 3 )
关键词
大黄
缺血
再灌注损伤
细胞凋亡
肠道屏障
Rheum officinale Baill
ischemiareperfusion injury
apoptosis
gut barrier
traditional Chinese drug
