摘要
目的探讨多药耐药(MDR)基因产物P-糖蛋白(P-gp)、谷胱甘肽-S-转移酶Ⅱ(GST-Ⅱ)、DNA拓扑异构酶(TOPO-Ⅱ)在肺癌组织中的表达及其与细胞核增殖抗原(Ki-67)相关性。方法采用免疫组织化学方法检测63例肺癌组织中P-gp、GST-Ⅱ、TOPO-Ⅱ和Ki-67的表达情况。结果 P-gp、GST-Ⅱ、TOPO-Ⅱ和Ki-67在肺癌组织中的阳性表达率分别为61.9%、63.5%、77.8%和39.7%。P-gp、GST-Ⅱ、Ki-67的表达与肺癌病理组织学分型、分化程度有关(χ2=6.733～22.532,P<0.01)。TOPO-Ⅱ在鳞癌和小细胞癌中的阳性表达率明显高于腺癌(χ2=8.259、4.189,P<0.01、0.05)。两个或两个以上MDR基因产物共表达率合计为90.5%,明显高于单独基因产物P-gp和TOPO-Ⅱ的表达率(χ2=59.660,P<0.01)。P-gp、GST-Ⅱ的表达与Ki-67之间无显著相关性(P>0.05),TOPO-Ⅱ的表达与Ki-67呈明显正相关(r=0.380,P<0.01)。结论 P-gp、GST-Ⅱ、TOPO-Ⅱ在不同肺癌类型中均有不同水平表达,且呈共同表达,它们的表达对肺癌的耐药起重要作用,因此联合检测有助于判断化疗疗效及预后。
Objective To explore the expression of products of multidrug resistance （MDR） gene-P glycoprotein （P-gp）, glutathione S epoxide transferase Ⅱ（GST Ⅱ ） and DNA topoisomerase （TOPO-Ⅱ ）-in human lung cancer and their correlation with Ki-67. Methods The expressions of P-gp, GST- Ⅱ , TOPO-Ⅱ and Ki-67 in 63 cases of lung cancer were detected by immu- nohistochemical method. Results The positive rates of P-gp, GST-Ⅱ , TOPO-Ⅱ and Ki-67 were 61.9 %, 63.5 %, 77.8 % and 39.7 %, respectively. The expressions of P-gp, GST-Ⅱ , and Ki-67 were correlated with the histological typing and differentiation of the cancer （X2 6. 733-22. 532,P〈0.01）. The expression rate of TOPO-Ⅱ in squamous cell carcinoma and small cell cancer was significantly higher than that in adenocarcinoma （Z2 =8. 259,4. 189;P〈0.01,0.05）. The coexpression rate of more than one MDR gene products was 90.5 G, which was apparently higher than the expression of single gene product such as P-gp and TOPOⅡ（Z 2 = 59. 660, P 〈0.01）. The expressions of P-gp and GST-Ⅱ were not obviously associated with that of Ki-67 （P 〉 0.05）, but that of TOPO Ⅱ was positively correlated with Ki-67 （r=0. 380,P〈0.01） Conclusion Different levels of expressions of P-gp, GST Ⅱ , and TOPO Ⅱcan be seen in different-type lung cancer, in pattern of co expression. Their expressions play an important role in lung cancer resistant to chemotherapy. Hence co detection of the expressions conduces to assessment of chemotherapeutic efficacy and prediction of prognosis.
出处
《齐鲁医学杂志》
2013年第1期11-13,16,共4页
Medical Journal of Qilu
基金
青岛市公共领域科技支撑计划项目(11-2-3-2-(3)-nsh)
