摘要
本文对红树内生细菌AiL3菌株的抑菌蛋白及其防治芒果炭疽病的效果与防病机理进行了研究。结果表明,AiL3菌株产生的抑菌物质主要为分子量28 kD的抑菌蛋白;60 g.mL 1抑菌蛋白处理芒果9 d后对炭疽病的防治效果达45.1%,与施保克的防治效果(43.6%)相当;抑菌蛋白抑制病原菌菌丝生长,导致菌丝现肿胀、膨大、畸形、细胞膜透性增加、细胞内含物外渗;抑菌蛋白处理后诱导芒果体内过氧化物酶(POD)活性下降,而苯丙氨酸解氨酶(PAL)活性上升。
An antifungal protein was separated and purified from endophytic bacterium AiL3 in mangrove Acanthus ilicifolius. The efficacy on mango anthracnose and bio-control mechanism was investigated. Results showed that the antifungal protein had strong antagonistic activity to Colletotrichum gloeosporioides. The molecular weight of antifungal protein from strain AiL3 was 28 kD. Control efficiency achieved 45.1% at 9 days after the antifungal protein treatment of concentration 60μg·mL^-1, which had no significant difference with that of Sportak （43.6%）. The antifungal protein could inhibit the hyphal growth and caused abnormal hyphal morphology of C. gloeosporioides, such as swollen, curliness and deformity. The treatment could also disrupt the structure of cell membrane, thereby induced the leakage of cytoplasm. The activities of peroxidase （POD） and phenylalanine ammonia-lyase （PAL） of mango after the treatment were decreased and increased, respectively.
出处
《中国生物防治学报》
CSCD
北大核心
2013年第1期104-109,共6页
Chinese Journal of Biological Control
基金
中国热带农业科学院中央级公益性科研院所基本科研业务费专项(sscri200711
sscri201002)
海南省自然科学基金(808183)
广东省科技计划(2009B020310015
2009B030803055)
关键词
内生细菌
抑菌蛋白
分离纯化
防治效果
防病机制
endophytic bacterium
antifungal protein
isolation and purification
control efficiency
bio-controlmechanism
