摘要
目的:探讨MPTP小鼠帕金森病(PD)模型脑内半胱氨酰白三烯受体CysLT1R、CysLT2R和GPR17表达分布特征。方法:C57BL/6小鼠连续5 d皮下注射MPTP 25 mg/kg诱导PD模型,第10 d处死动物。应用免疫组化方法,观察CysLT1R、CysLT2R和GPR17在黑质表达的变化,并用免疫双标方法进行细胞定位。结果:正常小鼠黑质中有CysLT1R、CysLT2R和GPR17表达,主要表达在TH阳性多巴胺能神经元以及小胶质细胞,CysLT2R还表达于星形胶质细胞;其中,在多巴胺能神经元中91%表达GPR17,77%表达CysLT1R,52%表达CysLT2R。与对照组比较,PD模型组小鼠黑质中TH阳性细胞数显著减少;同时CysLT1R、CysLT2R和GPR17阳性细胞数以及表达CysLT1R、CysLT2R和GPR17的多巴胺细胞数均显著减少。在正常小鼠纹状体中有CysLT1R、GPR17表达,主要表达于神经元;而CysLT2R表达很少,主要表达于星形胶质细胞。PD模型组小鼠纹状体中CysLT1R、GPR17阳性细胞数减少;但CysLT2R表达升高,主要表达于增生的星形胶质细胞。结论:CysLT1R、CysLT2R和GPR17可能参与MPTP所致的小鼠PD损伤过程。
Objective ： To examine the spatiotemporal profiles and localization of CysLTj R, CysLT2 R and GPR17 in mice with 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine （MPTP）-indueed Parkinson disease （PD）. Methods： PD model was induced by subcutaneous injection of MPTP （25 mg/kg） for 5 d in adult male C57BL/6 mice. At d10 after MPTP injection, the expression and cellular localization of CysLTIR, CysLT2 R and GPR17 in the substantia nigra were detected by immunohistochemistry and immunofluorescence. Results： CysLT1 R, CysLT2R and GPR17 were normally localized in TH-positive dopaminergic neurons and microglia, while CysLT2 R was also expressed in astrocytes. In dopaminergic neurons,approximately 91% co-expressed GPR17,77% co-expressed CysLT1R and 52% co-expressed CysLT2R. Compared with the control group, TH-positive cells in the substantia nigra were significantly reduced in PD mice. CysLTt R, CysLT2 R and GPR17-positive cells were significantly reduced; and CysLT1 R, CysLT2 R, GPR17-positive dopaminergic neurons were also significantly reduced in the PD group. In the striatum,both CysLT1R and GPR17 were normally expressed in neurons; whereas CysLT2R was expressed in astrocytes. In PD striatum, CysLT1 R and GPR17-positive cells were decreased, but CysLTiR expression was significantly increased which mainly expressed in the proliferating astrocytes. Conclusion： CysLT1 R,CysLT2Rand GPR17 may be involved in the MPTP-induced PD damage in mice.
出处
《浙江大学学报（医学版）》
CAS
CSCD
北大核心
2013年第1期52-60,共9页
Journal of Zhejiang University(Medical Sciences)
基金
国家自然科学基金项目(81072618)
浙江省自然科学基金项目(LY12H31010)
杭州师范大学国家项目培育基金(2010PYjj41)
关键词
半胱氨酸
受体
白三烯
受体
G-蛋白偶联
帕金森病
疾病模型
动物
免疫组织化学
Cysteine
Receptors, leukotriene
Receptors, G-protein-coupled
Parkinson disease
Disease models, animal
Immunohistochemistry
