摘要
目的探讨磁共振波谱成像（^1H-MRS）对肝纤维化早期诊断、量化分析及其分期的价值。方法CCl4法建立大鼠肝纤维化模型，成模大鼠40只及对照组7只进行MR波谱成像，成模大鼠按肝纤维化病理分期进行分组，分别计算并比较各组代谢物[胆碱（Cho）、谷氨酰胺及谷氨酸复合物（Glx）、乳酸（Lac）、肌酸（Cr）]与脂质（Lip）的峰高、波峰下面积的比值。结果（1）代谢物与脂质波峰峰高比值比较：Cho／Lip比值：对照组（0．05±0．03）与3期（0．40±0．30）、4期（0．44±0．30）比较P〈0．05；Glx／Lip比值：对照组（0．15±0．13）与2期（0．66±0．55）、3期（0．75±0．45）、4期（0．76±0．52）比较P〈0．05；Cr／Lip比值：对照组（0．14±0．27）与3期（0．92±0．58）比较P〈0．05。（2）代谢物与脂质波峰下面积比值比较：Cho／Lip比值：对照组（0．12±0．13）与4期（0．47±0．37）比较P〈0．05；Glx／Lip比值：对照组（0．05±0．04）与1期（0．54±0．32）、2期（0．45±0．36）、3期（0．48±0．40）、4期（0．53±0．34）比较P〈0．05；Cr／Lip比值：对照组（0．11±0．23）与4期（0．81±0．71）比较P〈0．05。各组Lac／Lip波峰峰高及面积比值比较P〉0．05。结论Cho／Lip、Glx／Lip、Cr／Lip波峰峰高比值、波峰下面积比值对肝纤维化分期具有一定的价值。
Objective To explore the values of magnetic resonance spectrum （MRS） in early diagnosis, quantization analysis and staging of hepatic fibrosis. Methods A rat model of hepatic fibrosis was established by the method of carbon tetracarbon （ CCl＋ ）. A total of 47 SD rats were divided into model （n =40） and control （n = 7） groups. 1H-MRS was performed. The model rats of hepatic fibrosis were grouped according to their pathological stages. The ratio of peak height and peak area of metabolites and lipid （ Cho/Lip, Glx/Lip, Lac/Lip and Cr/Lip） were calculated and compared respectively. Results The ratios of peak height of metabolites and lipid were as follows： ratio of Cho and Lip： significant differences existed between control and grades 3 and 4 model groups （P 〈 0. 05 ） ; ratio of Glx and Lip ： significant differences existed between control and grades 2, 3 and 4 model groups （ P 〈 0.05 ） ; ratio of Cr and Lip ： significant differences existed between control and grade 3 model groups （ P 〈 0. 05 ）. The peak area ratio of main metabolites and lipid of liver were as follows ： ratio of Cho and Lip ： significant differences existed between control and grade 4 model groups （ P 〈 0. 05 ） ; ratio of Glx and Lip ： significant differences existed between control and other groups （P 〈 0. 05 ） ; ratio of Cr and Lip ： significant differences existed between control and grade 4 model groups （ P 〈 0. 05） ; ratio of Lac and Lip ： no significant differences existed between these groups （P 〉 0.05）. Conclusion The ratios of peak height and peak area of Cho/Lip, Glx/Lip and Cr/Lip are important for the staging of hepatic fibrosis.
出处
《中华医学杂志》
CAS
CSCD
北大核心
2013年第5期376-379,共4页
National Medical Journal of China
基金
江苏省苏州市应用基础研究计划（SYS201041）
苏州大学附属第二医院博士、留学归国人员预研项目（SDFEYBSll07）
关键词
磁共振波谱学
肝硬化
大鼠
Magnetic resonance spectroscopy
Liver cirrhosis
Rats
