摘要
目的检测类风湿关节炎（RA）患者外周血白细胞介素（IL）．32、肿瘤坏死因子（TNF）-a的表达水平，同时分析其与c反应蛋白（CRP）、红细胞沉降率（ESR）、类风湿因子（RF）和疾病活动度积分（DAS28）的关系，初步探讨IL-32在RA的临床意义。方法收集97例RA患者、36例非RA结缔组织病患者及30名健康志愿者外周血标本，其中RA高活动期36例、RA中低活动期33例、RA缓解期28例。采用实时荧光定量．聚合酶链反应（QRT-PCR）检测外周血单个核细胞（PBMC）中的IL-32和TNF．a的mRNA的水平，酶联免疫吸附实验（EusA）检测血浆IL-32、TNF．o【的蛋白浓度，并分析其与临床指标的关系。采用单因素方差分析、Spearnlan相关分析和多元回归分析进行统计学处理。结果①活动期RA患者PBMC中IL-32和TNF．Ot的mRNA的表达水平高于非RA结缔组织病和健康对照组（RA组高活动期、中低活动期与健康对照组的IL-32水平分别为0．29＋0．07，0．24±O．06与0．11＋0．06；TNF-ct分别为0．042~0．022，0．012±0．006与0．004m0．002，P均〈0．01）；且RA活动期高于RA缓解期（P〈0．01）。②血浆IL-32和TNF．a蛋白水平也显示类似的结果：RA活动组明显高于RA缓解组和对照组（RA组高活动期、中低活动期与健康对照组的IL-32水平分别为[（107~42），（66＋27），（20＋10）pg／ml；TNF—Ot分别为（261±77），（186±84），（72＋20）pg／m1，P〈0．01]。③RA患者组外周血IL-32的表达水平与TNF．仪，CRP，ESR，RF以及疾病活动度积分（DAS28）呈正相关（r值分别为0．568，0．321，0．185，0．325，0．303，P〈0．01）。RA患者外周血IL-32的mRNA水平及蛋白水平与TNF．仅相应水平具有相关性（b=1．721，P〈0．01及b=0．176，P〈0．01）。结论外周血IL-32的水平有可能作为RA活动性参考指标之一。
Objective To detect the expression levels of interleukin（lL）-32 and tumor necrosis factor （TNF）-ot in peripheral blood of rheumatoid arthritis （RA） patients, and analyze the relations between IL-32 and level of erythrocytes （ESR）, ereactive protein （CRP）, rheumatoid factor （RF）, the disease activity index 28 （DAS28） scores in RA patients. The aim of this study is to identify the clinical significance of IL-32 in RA patient. Methods We collected 97 specimens of peripheral blood from RA patients, including 36 RA patients with high active disease, 33 RA patients with moderate active disease, 28 RA patients with stable disease, 36 non-RA connective tissue diseases （CTD） patients and 30 healthy volunteers. The mRNA levels of IL-32, TNF-ot in the peripheral blood mononuclear cells （PBMC） were determined by quantitative real-time PCR （QRT-PCR）. The concentration of IL-32, TNF-ct in plasma were detected by enzyme hnked immunosorbent assay （ELISA）, one-way ANOVA and Spearmah＇s correlation and multiple linear regression were adopted for statistical analysis. Results （1）The mRNA levels of IL-32 and TNF-ot in active RA patients were higher than those in healthy control group （ 0.29~0.07, 0.24＋0.06 vs 0.11＋0.06 for IL-32 in the high active RA group and moderate active RA group versus the healthy group; and 0.042＋0.022, 0.012＋0.006 vs 0.004~0.002 for TNF-ct in the high active RA group and moderate active RA group versus the healthy group,P〈0.01 ）, and than those in stable RA patients, and non-RA CTD patients. There was no significant difference among the stable disease groups, healthy control group and non-RA CTD group in the level of mRNA. （2） The concentration of IL-32, TNF-a in the plasma of RA patients with active disease was higher than that of healthy control group [（107_＋42） pg/ml, （66_＋27） pg/ml vs （20_＋10） pg/ml for IL-32 in the high active RA group and moderate active RA group versus the healthy group; and （261_＋77） pg/ml, （186_＋84） pg/ml vs （72_＋20） pg/ml for TNF-ot in the high active RA group and moderate active RA group versus the healthy group, P〈0.O1 ].＂ （~） The expression levels of IL-32 was positively correlated with the level of TNF-ct, ESR, CRP, RF, DAS28 scores in the plasma of RA patients （r=0.568, 0.321, 0.185, 0.325, 0.303 respectively, P〈0.01）. （4）The expression levels of IL-32 was positively correlated with the level of TNF-ct （the regression coefficients and P values were b=1.721, P〈0.01 and b=0.176, P〈0.01 respectively）. Conclusion The levels of IL-32 in peri- nheral blood may be used as an indicator for disease activity of RA.
出处
《中华风湿病学杂志》
CAS
CSCD
北大核心
2013年第2期103-108,共6页
Chinese Journal of Rheumatology
