摘要
目的:研究SGC7901细胞与GES-1细胞中食管癌相关基因4(ECRG4)蛋白表达差异及5-杂氮-2′-脱氧胞苷(5-Aza-CdR)干预前后SGC7901细胞中ECRG4基因表达变化情况。方法:Westernblot检测SGC7901细胞、GES-1细胞中ECRG4编码蛋白的表达,以不同浓度(0、5、10μmol/L)5-Aza-CdR干预SGC7901,48h后再以Westernblot检测ECRG4编码蛋白的表达情况。结果:SGC7901细胞中ECRG4蛋白表达较低而GES-1细胞中表达较高,差异有统计学意义(P<0.001)。以5-Aza-CdR干预SGC7901细胞48h,随着药物浓度增加,ECRG4蛋白表达逐渐上调,10μmol/L处理组比5μmol/L组更明显,差异有统计学意义(P<0.01)。结论:SGC7901细胞中ECRG4蛋白的表达量明显低于GES-1细胞;以5-Aza-CdR处理SGC7901细胞48h后ECRG4蛋白表达有上调,且10μmol/L组比5μmol/L组其恢复表达作用更明显。
Objective To study the difference of ECRG4 protein expression between SGC7901 cells and GES-1 cells and the changes of ECRG4 gene expression in SGC7901 before and after 5-Aza-CdR intervention. Methods Western blot was used to detect ECRG4 protein expression in SGC7901 and GES-I. SGC7901 cells were then intervented by 5-Aza-CdR at different concentrations （0, 5, and 10 μmol/L）. 48 h after intervention, Western blot was used again to determine ECRG4 protein expression. Results ECRG4 protein expressions were lower in SGC7901 and higher in GES-I, with a statistical significant difference （P 〈 0.001）. 48 h after intervetion with 5-Aza-CdR for SGC7901, with an increase in drug concentration, ECRG4 protein expression upregulated gradually; the change was more obvious in the 10μmol/L group than in the 5 μmol/L group, with a statistical difference （P 〈 0.01 ）. Conclusions The quantity of ECRG4 protein expression is smaller in SGC7901 cells than in GES-1 cells. 48 h after treatment with 5-Aza-CdR, ECRG4 protein expression is upregulated in SGC7901, and the upregulation is more significant in the 10 μmol/L group than in the 5 μmol/L group.
出处
《实用医学杂志》
CAS
北大核心
2013年第3期346-348,共3页
The Journal of Practical Medicine
基金
湖南省教育厅项目(编号:10C0956)
湖南省自然科学基金项目(编号:11JJ5068)
