摘要
目的:研究上呼吸道NK/T细胞淋巴瘤的病理及免疫表型特征,探讨EB病毒(EBV)感染和上呼吸道NK/T细胞淋巴瘤发生、发展的关系。方法:采用免疫组织化学方法检测47例上呼吸道NK/T细胞淋巴瘤中有关免疫表型CD20、CD3、CD79a、细胞毒颗粒相关抗原-1(TIA-1)、CD56、CD45RO、颗粒酶B(Granzyme B),以此确定上呼吸道NK/T细胞淋巴瘤细胞免疫表型及EBV潜伏蛋白(LMP-1),原位杂交技术检测EBV编码的RNA(EBER 1/2)。结果:CD3、CD56、CD45RO、Granzyme B阳性表达率达100.0%,EBER阳性表达率达87.2%;LMP-1阳性表达率为40.4%。结论:感染EBV与上呼吸道NK/T细胞淋巴瘤的发生、发展有密切关系。
Objective： To study the immunophenotypic features of the upper respiratory tract NK/ T cell lymphoma and its association with Epstin-Barr virus （EBV） infection. Methods： LCA,CD20,CD3, CD79a,CD56,CD45RO,TIA-1,Granzyme B in 47 cases of NK/T cell lymphoma were detected by immuno- histochemistry staining method. The expression of EBV-latent membrane protein （LMP-1） were detected by immunohistochemistry, EBV-encoded RNA （EBER 1/2） were detected by in situ hybridization. Re- suits： Among these cases, LCA, CD3,CD56, CD45RO,TIA-1, Granzyme B were positive in the 47 cases （100%）. The positive rates of EBER 1/2 and LMP-1 were 82.3%and 40.4%. Conclusion： The occur- rence and progression of NK/T cell lymphoma is closely associated with EBV infection.
出处
《海南医学院学报》
CAS
2013年第1期21-24,共4页
Journal of Hainan Medical University
基金
中国高校医学期刊临床专项资金项目(112210468)~~
关键词
上呼吸道
NK
T淋巴瘤
EB病毒
免疫组化
原位杂交
Upper respiratory NK/T lymphoma Epstin-Barr virus immunohistochemistry Insitu hybridization
