摘要
本实验以抗旱性不同的小麦"宁春4号"和"西旱3号"材料,研究不同浓度NaCl处理对其幼苗渗透性调节物含量及脯氨酸代谢相关酶活性的影响.50mmol.L-1处理使宁春4号叶、根可溶性蛋白及根可溶性糖的含量较对照显著增加,而150mmol.L-1处理下两种小麦叶和根可溶性糖、可溶性蛋白和脯氨酸含量均增加.此外,150mmol.L-1处理使宁春4号小麦叶和根谷氨酸激酶(GK)活性的增幅明显高于鸟氨酸转氨酶(OAT),且相比对照差异显著,而西旱3号小麦叶OAT活性和根GK活性较对照显著增强.50mmol.L-1盐处理使两种小麦叶和根脯氨酸脱氢酶(PDH)活性较对照均明显下降,150mmol.L-1 NaCl处理只对西旱3号叶和根PDH活性有明显的抑制作用.总之,盐胁迫诱导两种小麦可溶性糖、可溶性蛋白及脯氨酸的积累,这有助于增强植物的耐盐性;150mmol.L-1盐处理下下宁春4号叶、根中脯氨酸的积累可能与GK活性的增加有关,西旱3号叶脯氨酸的积累与OAT活性的升高及PDH活性的下降有关,而西旱3号根脯氨酸的积累可能是GK活性升高及PDH活性降低的结果.
The aim of this study is to investigate the changes of osmolyte content and proline metabolism enzyme activity in response to different NaCl concentrations in two wheat seedlings,cv Ningchun 4 and Xihan 3.Compared with the control,the amount of soluble protein in Ningchun shoots and roots as well as soluble sugar content in the roots increases remarkably exposed to 50 mmol·L-1 NaCl stress,while soluble sugar,soluble protein and proline contents enhance in the shoots and roots of two wheat with 150 mmol·L-1 NaCl-treatment.In addition,the increased rate of glutamate kinase（GK） activity is significantly higher than that of ornithine aminotransferase（OAT） activity in Ningchun shoots and roots treated with 150 mmol·L-1 NaCl,while the GK activity in the shoots and OAT activity in the roots of Xihan seedlings elevate significantly as compared with the control.Proline dehydrogenase（PDH） activity significantly decreases in the shoots and roots of two wheat with 50 mmol·L-1 NaCl-treatment,while this enzyme activity in the shoots and roots of Xihan are inhibited under 150 mmol·L-1 NaCl-treatment.In conclusion,salt stress induces the accumulation of soluble sugar,soluble protein and proline in two wheat seedlings,which contributes to the enhancement of plant salt tolerance;the accumulation of proline may be associated with increased GK activity in Ningchun shoots and roots under 150 mmol·L-1 NaCl concentration,but associated with the stimulation of OAT and the inhibition of PDH in Xihan shoots,and increased GK activity and decreased PDH activity may induce to the proline accumulation in the roots of Xihan.
出处
《西北师范大学学报（自然科学版）》
CAS
北大核心
2013年第1期72-77,91,共7页
Journal of Northwest Normal University(Natural Science)
基金
国家自然科学基金资助项目(31160088)
教育部新世纪优秀人才支持计划项目
