摘要
目的确定口岸蝇类携带病原菌种类及分布。方法将蝇类体表、体内细菌分离培养,单克隆菌株经Vitek 2 Compact微生物自动分析及16SrRNA基因序列比对。结果在7种蝇体表检出18种菌,其中致病菌2种,条件致病菌14种,包括1株国内未报道的条件致病菌株Wohlfahrtiimonas chitiniclastica;蝇体内检出14种菌,其中致病菌1种,条件致病菌12种;携带菌种类最多的是酱麻蝇,其次为亮绿蝇。结论口岸蝇体携带病原菌的调查,有利于对新型病原微生物入侵的鉴别及应对突发公共卫生事件。
Objective To investigate the species and distributions of fly-borne pathogens in Shanghai Pudong International Airport.Methods The bacteria on the body surfaces and inside the bodies of flies were isolated and cultured.The monoclonal strains were subject to automatic analysis using Vitek 2 Compact and 16S rRNA sequence alignment.Results Eighteen species of bacteria（2 pathogens and 14 opportunistic pathogens including Wohlfahrtiimonas chitiniclastica that had not been reported in China） were detected on the body surfaces of 7 species of flies,Fourteen species of bacteria（1 pathogens and 12 opportunistic pathogens） were detected inside the bodies of the flies.The fly that carried the most species of bacteria was Sarcophaga misera,followed by Lucilia illustris.Conclusion The investigation of fly-borne pathogens in port will be in favor of the identification of new pathogen species and response to public health emergencies.
出处
《中国媒介生物学及控制杂志》
CAS
CSCD
2012年第6期506-511,共6页
Chinese Journal of Vector Biology and Control
基金
国家质检总局科技计划项目(2011IK153)
中国检验检疫科学研究院所基金(2011JK007)~~
关键词
浦东机场
口岸
蝇类
病原菌
16SrRNA
鉴定
Pudong International Airport
Port
Fly
Pathogen
16S rRNA
Identification
