摘要
目的:观察准噶尔乌头及其炮制品对佐剂性关节炎(AA)大鼠的作用,并探讨其作用机制。方法:设正常对照组、模型对照组、阳性药对照组(醋酸泼尼松片6 mg/kg)、准噶尔乌头及其炮制品(包括按药典方法、文献方法、哈萨克族方法 3种炮制品)高、中、低剂量组(剂量分别为30.65,15.32,7.66 mg/kg);除正常对照组外,其余各组均采用弗氏完全佐剂(FCA)诱导大鼠AA模型;各组于造模致炎当天灌胃给药,连续23 d;观察各药对AA大鼠踝关节足肿胀程度和关节炎指数的影响;采用ELISA法检测各组血清中白介素-1β(IL-1β)和皮质醇(Cort)含量。结果:(1)模型组大鼠踝关节足肿胀度高于正常组(P<0.01),与模型组比较,准噶尔乌头及其炮制品各给药组踝关节足肿胀度和关节炎指数均显著降低(P<0.01或P<0.05)。(2)与正常组比较,模型组大鼠血清IL-1β、Cort水平均高于正常组(P<0.01);与模型组比较,阳性组、准噶尔乌头生品高、中、低剂量组及药典法炮制品高、中剂量组和文献法炮制品高剂量组的大鼠血清Cort水平均降低(P<0.01);与模型组比较,阳性组、准噶尔乌头生品和药典法炮制品高、中、低剂量组及文献法炮制品高剂量组、哈萨克族法炮制品中剂量组的IL-1β水平均降低(P<0.01或P<0.05)。结论:准噶尔乌头及其炮制品具有抗炎作用,其机制可能是通过抑制炎症细胞因子IL-1β、Cort治疗佐剂性关节炎大鼠。
Objective： To observe the effect of Aconitum soongaricum and its processed products on adjuvant arthritis（AA） rat and its mechanism.Methods： Rats were radomly divided into normal group,model group,positive group,Aconitum soongaricum and its processed products（including Pharmacopoeia method,document method,Kazak method three processed products）,which divided into high dose,middle dose,low dose groups（30.65,15.32,7.66 mg/kg）;Except normal group,prepared AA rat model by using Freund＇s complete Adjuvant（FCA） for the rest groups;Each group was administered for 23 days after the rat model was established,observed rats ankle swelling degree and arthritis index.Determined content of interleukin-1β（IL-1β） and cortisol（Cort） in serum by ELISA method.Results：（1）The swelling degree of model group was higher than that of normal group（P0.01）.Compared with model group,the swelling degree and AI of Aconitum soongaricum and all of its processed products groups were lower（P0.01 or P0.05）.（2）The content of IL-1β and Cort in serum of model group was higher than that of normal group（P0.01）.Compared with model group,the content of Cort in serum of positive group,Aconitum soongaricum groups（high dose,middle dose,low dose）,its Pharmacopoeia method processed product groups（high dose,middle dose）and its document method processed product groups（high dose） were lower（P0.01）;Compared with model group,the content of IL-1β in serum of positive group,Aconitum soongaricum groups（high dose,middle dose,low dose）,its Pharmacopoeia method processed product groups（high dose,middle dose,low dose） its document method processed product group（high dose）and its Kazak method processed product group（middle dose） were lower（P0.01 or P0.05）.Conclusion： Aconitum soongaricum and all of its processed products groups are proved with anti-inflammatory effect,which possible mechanism would be restraining inflammation cell factor such as IL-1β and Cort in serum of AA rat.
出处
《中药材》
CAS
CSCD
北大核心
2012年第10期1572-1576,共5页
Journal of Chinese Medicinal Materials
基金
自治区自然科学基金项目(2011211Ax092)
自治区中医药管理局中医民族医药青年科技人才专项课题(2010ZMY03)
国家自然科学基金项目(30960514)
关键词
新疆准噶尔乌头
炮制品
佐剂性关节炎
白介素1Β
皮质醇
Aconitum soongaricum Stapf.
Processed product
Adjuvant arthritis
Interleukin-1β
Cortisol
