摘要
目的观察5-羟色胺7(5-HT7)受体在便秘及腹泻模型大鼠肠道中的表达差异,探索其对肠道运动的调节作用。方法用冰水灌胃法制备便秘大鼠模型,束缚刺激方法制备腹泻模型并设对照组。观察各组胃肠通过时间、粪便形状,用免疫组化及实时定量PCR法测定各组大鼠肠道组织中5-HT7受体阳性细胞数及基因表达差异。结果便秘组大鼠粪便特征及胃肠通过时间显示胃肠运动迟缓特征;而腹泻组粪便特征及胃肠通过时间显示胃肠运动过速特征。便秘组回肠及结肠组织中5-HT7受体阳性细胞数及基因表达高于对照组(P<0.01),并且结肠组织中高于腹泻组(P<0.01)。结论 5-HT7受体参与结肠平滑肌松弛及结肠移行性复合运动(CMMC)的发生及传播的调节,便秘型大鼠肠道运动紊乱与肠道5-HT7受体表达增高可能相关。
Objective To observe the expression of 5-hydroxytryptamine （5-HT7） receptor in the intestinal tract of rat constipation and diarrhea models so as to probe the role of 5-HT7 receptor in modulation of intestinal motility. Methods Rat model of constipation was established by gastric irrigation with cool water. Rat model of diarrhea was established by restrain stress while the contorl rats were not intervened. After gastrointestinal transiting time and feces properties were surveyed, the expression of intestinal 5-HT7 receptor was investigater by immunohis- tochemistry and realtime quantitative PCR. Results The feces properties of constipation were less and drier than those of the controls, and the gastrointestinal transiting time in constipation was longer. The number of positive cells and mRNA expression of 5-HT7 receptor in the ileum and colon of the constipation rats were higher than those of the controls （P〈0.01）, and higher in the colon of the constipation rats than in the diarrhea group （P〈0.01）. Conclusion 5-HT7 receptor plays a role in modulating gastrointestinal tract contractility and the colonic migrating motor complex （CMMC）. The gastrointestinal dyskinesis in constipation rats may be associated with a higher expression of 5-HT7 receptor in the intestines.
出处
《西安交通大学学报（医学版）》
CAS
CSCD
北大核心
2013年第1期34-37,共4页
Journal of Xi’an Jiaotong University（Medical Sciences）
关键词
5-HT7
受体
腹泻
便秘
平滑肌收缩
结肠移行性复合运动
5-hydroxytryptamine （5-HTT） receptor
diarrhea
constipation
smooth muscle contractility
colonic migrating motor complex
