摘要
目的 探讨酶免疫测定法检测幽门螺杆菌粪便抗原 (HpSA)在诊断幽门螺杆菌 (Hp)感染中的价值。方法 用酶免疫测定法检测 6 3例患者粪便标本HpSA ,其中 6 1例同时进行1 3C 尿素呼气试验 (1 3C UBT) ,且 31例患者也采用组织学方法进行检查。以1 3C UBT作为金标准 ,对酶免疫测定法和组织学方法进行初步评价。结果 酶免疫测定法的敏感性为 94.7% ,特异性为 95 .7% ,准确性为 95 .1% ,阳性预测值为 97.3% ,阴性预测值为 91.7%。均高于组织学方法。结论 酶免疫测定法检测HpSA具有较高的敏感性和特异性 ,方法简便、取材方便 ,适合普通实验室作为常规项目开展。此法既适合幽门螺杆菌感染的筛选与诊断 ,也可用于治疗后是否根治的监测。
Objective To evaluate the clinical utility for the diagnosis of Helicobacter pylori (Hp) infection by enzyme immunoassay (EIA) for detecting Hp stool antigen (HpSA) in feces.Methods HpSA in stool specimens of 63 patients were detected by EIA. 61 of the 63 patients also underwent 13 C Urea breath test( 13 C UBT).The tissue samples of 31 patients were obtained endoscopically and examined with histologic technique (Warthin Starry silver stain). 13 C UBT was regarded as a gold standard, and EIA and histologic techniques were evaluated.Results The sensitivity and specificity of EIA were 94.7% and 95.1% respectively; the positive and negative predictive values were 97.3% and 91.7% respectively; and the accuracy was 95.1%. These indexes of EIA were higher than those of histologic technique.Conclusion EIA foe detecting HpSA in feces is a rapid, noninvasive Both have high sensitivity and high specificity. It is suitable for the screening and diagnosis of Hp infection, and monitoring the effectiveness of treatment.
出处
《中华检验医学杂志》
CAS
CSCD
2000年第3期150-152,共3页
Chinese Journal of Laboratory Medicine
关键词
粪便
幽门螺杆菌
抗原
酶免疫法
Immunoenzymatic techniques
Helicobacter pylori
Feces
