摘要
为建立一种快速测定蔬菜中毒死蜱的新方法,以韭菜、苋菜、黑白菜和红薯叶为样品,基于样品中残留毒死蜱与结晶紫-碘化钾在pH11.00的B-R缓冲溶液中形成新的缔合物,利用共振光散射技术进行测定。结果表明:其最大共振光散射峰位于650nm波长处,在0.016～0.877μg/mL范围内毒死蜱质量浓度与体系的相对散射光强度(I)有良好的线性关系,相关系数r为0.9981,检出限为0.016μg/mL;样品测定的RSD不大于2.5%(n=5),回收率为91.4%～100.3%。方法快速简便、灵敏度较高,用于蔬菜中毒死蜱残留量的测定,结果满意。
A new resonance light scattering（RLS） method for the rapid determination of residual chlorpyrifos in vegetables such as Chinese leek,amaranth,sweet potato,and black cabbage was developed based on the reaction of chlorpyrifos with crystal violet-potassium iodide in Britton-Robinson butter at pH 11.00 to generate an ion association complex.Chlorpyrifos had maximum resonance light scattering peak at 650 nm.A good liner relationship（r = 0.9981） was observed between chlorpyrifos concentration over the range of 0.016–0.877 μg/mL and relative scattered light intensity.The detection limit of chlorpyrifos was 0.016 μg/mL and the relative standard deviations（RSDs） of precision for 5 replicate determinations were less than 2.5%.The average recovery rates of chlorpyrifos in four vegetables were 91.4%–100.3%（n = 5）.This method was simple,rapid and sensitive and satisfying results were obtained in its practical applications.
出处
《食品科学》
EI
CAS
CSCD
北大核心
2012年第22期238-241,共4页
Food Science
基金
河南省重点学科建设项目(10466-X-082301)
关键词
共振光散射
毒死蜱
结晶紫-碘化钾
蔬菜
resonance light scattering
chlorpyrifos
crystal violet-potassium iodide
vegetables
