摘要
建立了K3[(Fe(CN)6]-Fe(Ш)分光光度法测定卡托普利的新方法。结果表明,卡托普利分子中的巯基(-SH)可将Fe(III)还原为Fe(II),还原生成的Fe(II)与K3[Fe(CN)6]反应生成可溶性普鲁士蓝KFe[Fe(CN)6],通过测定可溶性普鲁士蓝的吸光度,从而间接测定卡托普利的含量。显色体系的最大吸收波长为710 nm,卡托普利在2.12～19.1μg/mL范围内与吸光度A呈良好的线性关系,线性回归方程为A=0.0087+0.0276ρ(μg/mL),线性相关系数r=0.9993。方法用于实际药品中卡托普利的含量测定,结果与药典法一致。
A novel method for the spectrophotometric determination of captopril using potassium ferricyanide-Fe（Ш） has been established.The results show that Fe（Ш） can be reduced to Fe（Ⅱ） by hydrosulfuryl（-SH） in captopril molecule,and then Fe（Ⅱ） reacts with K3[Fe（CN）6] to form soluble prussian blue（KFe[Fe（CN）6]）,and the content of captopril is determined indirectly through determining the absorbance of the soluble prussian blue.The maximum absorption wavelength of chromogenic system is 710 nm,good linear relationship is obtained between the absorbance and the concentration of captopril in the range of 2.12-19.1 μ g/mL,and the linear regression equation is A=0.0087＋0.0276ρ（μg/mL）,with a linear correlation coefficient of 0.9993.This proposed method has been applied to the determination of captopril in real pharmaceuticals,and the results agree well with those by pharmacopoeial method.
出处
《分析试验室》
CAS
CSCD
北大核心
2012年第12期89-91,共3页
Chinese Journal of Analysis Laboratory
