摘要
目的探讨Survivin和Ang2在人乳腺癌组织中的表达及其在淋巴转移中的作用。方法 60例乳腺癌组织,应用免疫组织化学S-P法检测Survivin和Ang2在乳腺癌组织中的表达情况,RT-PCR检测Survivin及VEGF-CmRNA的表达。结果 60例乳腺癌组织中Survivin和Ang2的表达阳性率分别为85.0%和73.3%,Survivin阳性表达组中Ang2的表达为74.5%(35/51),Survivin阴性表达组中Ang2的表达为66.7%(6/9),经直线相关分析,两者在乳腺癌中的相关系数r=0.32,呈正相关,P=0.01。结论 Survivin和Ang2在乳腺癌组织中过表达,呈正相关。Survivin表达上调可能促使Ang2的过表达,诱导乳腺癌淋巴管生成,从而导致乳腺癌淋巴转移的发生。
Objective To investigate survivin and Ang2 expressions in breast carcinoma tissue and its effect in lymphatic metastasis.Methods 60 cases of breast carcinoma,Immunohistochemical SP method was adopted in detecting survivin and Ang2 expressions in breast carcinoma tissue,RT-PCR for detecting survivin and VEGF-C mRNA expressions.Results Positive rate of survivin and Ang2 was 85.0% and 73.3% respectively in 60 cases of breast carcinoma,positive rate of Ang2 was 74.5%（35/51） in the positive Survivin expression group,while that in negative Survivin expression Group was 66.7%（6/9） respectively,with undergoing correlation analysis,both sides were positive correlated with each other in breast carcinoma（r=0.32,p=0.001）.Conclusion The expressions of survivin and Ang2 were high in breast carcinoma,which were positively correlated with each other.Up-regulation in survivin expression may urge hyper-expression of Ang2,accordingly to promote tumor lymph angiogenesis and metastasis of tumor lymph node.
出处
《中国实验诊断学》
2012年第11期2014-2017,共4页
Chinese Journal of Laboratory Diagnosis
基金
山东省自然基金项目(ZR2009CM039)
山东省医药卫生科技发展计划(2011HW084)
