摘要
以豫杂一号泡桐花药为材料,开展了其花药愈伤组织诱导的研究.结果表明,外植体的消毒方式以体积分数70%乙醇处理30 s后再用体积分数0.1%HgCl2消毒60 s最为理想;4℃预处理有利于花药愈伤组织的诱导,以低温处理7 d的花药愈伤组织诱导率为最高;30 g.L-1蔗糖质量浓度诱导花药愈伤组织的效果较好,诱导率达58.76%;诱导愈伤组织以MS培养基附加30 g.L-1蔗糖+2 mg.L-1NAA+3 mg.L-16-BA为最佳.
Abstract： Callus induction from anthers of Paulownia tomentosa × Paulownia fortune was studied in this paper. The results showed that it was an ideal disinfection way to soak explants in 70% alcohol for 30 s first and then in 0.1% HgC12 for 60 s. The rate of callus induction at4 ℃ temperature for 7 days can reached the highest. The 3% sucrose concentration was suitable for the induction, and the rate of induction reached 58.76%, The best medium to induce calli from anthers is MS medium ＋ 30g·L^-1 sucrose ＋ 2 mg·L^-1 NAA ＋ 3 mg·L^-1 6-BA.
出处
《河南农业大学学报》
CAS
CSCD
北大核心
2012年第5期530-534,共5页
Journal of Henan Agricultural University
基金
河南省高校杰出人才工程项目(2001)
关键词
豫杂一号泡桐
花药培养
愈伤组织
诱导
Paulownia tomentosa x Paulownia fortunei
anther culture
callus
induction
