摘要
噬菌体随机肽库技术是将编码外源多肽的DNA序列插入到噬菌体外壳蛋白结构基因的适当位置,使外源基因随外壳蛋白结构基因的表达而表达,被展示的外源多肽可保持相对独立的空间结构和生物活性,是一种基因型与表达型的统一。近年来,噬菌体随机肽库技术被广泛应用于各种抗原的模拟表位筛选中,并取得了显著成果。综述了利用噬菌体随机肽库技术在抗原模拟表位筛选中的研究进展。
In this paper, the screening mimicking epitope of antigen from phage random peptide library was reviewed. The phage random peptide library is a technology by inserting the DNA sequences of polypeptide into the phage coat protein gene at the proper position and the heterolo- gous gene is expressed accompanied with the coat protein. Then,the displayed heterologous poly- peptide can be kept relatively independent spatial structure and biological activity,which is a unity of genotype and phynotype. In recent years, phage random peptide library has been widely used for screening the mimicking epitope of various antigen and has made remarkable achievements.
出处
《河南农业科学》
CSCD
北大核心
2012年第10期14-18,共5页
Journal of Henan Agricultural Sciences
基金
农业部公益性行业(农业)科研专项(201203040)
河南省农业科学院科技发展及示范推广专项
关键词
噬菌体随机肽库
噬菌体展示技术
模拟表位
抗原
phage random peptide library
phage display technology
mimicking epitope
antigen
