摘要
目的:建立蝎龙接骨散中羟基红花黄色素A和阿魏酸的测定方法。方法:采用反相高效液相色谱法法进行定量分析,用Inertsil C8-3柱,乙腈-0.2%磷酸-四氢呋喃(28∶70∶2)为流动相,流速1.0 mL·min-1,检测波长340 nm。结果:羟基红花黄色素A和阿魏酸的线性范围分别为11.13～111.35 mg·L-1和3.42～34.2 mg·L-1;平均加样回收率分别为100.35%,99.23%。结论:此法简便、准确,具有良好的重复性和回收率,适用于蝎龙接骨散中羟基红花黄色素A和阿魏酸的同时测定。
Objective： To establish a method for determination of the content of hydroxysafflor yellow A and ferulic acid in Xielongjiegu powder. Method： The quantitative analysis was carried out by HPLC with a Inertsil C8.3 column. The mobile phase was acetonitrile-0. 2% phosphoric acid-tetrahydrofuran （28： 70： 2） with a flow rate at 1.0 mL .min-1 Detection wavelength was set at 340 nm. Result： The linear ranges for hydroxysafflor yellow A -1 and ferulic acid were 11.13-111.35 mg-L-1 and 3.42-34.2 mg. L respectively. The average recoveries were 100.35% and 99.23%. Conclusion： This method is simple and accurate with good repeatability and recovery. It is suitable for the determination of the content of hydroxysafflor yellow A and ferulic acid in Xielongjiegu powder.
出处
《中国实验方剂学杂志》
CAS
北大核心
2012年第22期109-111,共3页
Chinese Journal of Experimental Traditional Medical Formulae
基金
广西科技厅攻关基金项目(9920028)
广西柳州市科技局科技攻关基金项目(990608)
