摘要
目的:考察从石见穿醇提物氯仿部位提取分离得到的熊果酸对5株人肿瘤细胞株的体外增殖抑制作用,为开发新的抗癌药物提供理论依据。方法:使用95%工业酒精对石见穿饮片进行提取,然后分别用石油醚、乙酸乙酯、氯仿和正丁醇依次对乙醇提取部位进行分段萃取,采用系统溶剂法、梯度洗脱、柱色谱分离、重结晶等方法,从石见穿醇提物氯仿部位中分离得到熊果酸。采用MTT法测定熊果酸对体外培养的5株人肿瘤细胞株的增殖抑制作用。结果:在体外,熊果酸对于5株细胞株具有较强的增殖抑制作用。结论:熊果酸是石见穿醇提物、氯仿部位发挥体外抗肿瘤作用的主要有效成分之一。
Objective：To investigate in vitro anticancer activities of ursolic acid isoluted from salvia chinensis beth.Methods：The extraction from salvia chinensis beth.with 95% ethanol and then from the ethanol extraction part in turn with petroleum ether,ethyl acetate,trichloromethane and n-butanol was conducted.The means of systematic solvent extraction,gradient elution,column chromatography and recrystallization were used to separate the trichloromethane extraction part from salvia chinensis beth.The inhibitory effects of ursolic acid on 5 human tumor lines were measured by MTT assay.Results：The ursolic acid isoluted from salvia chinensis beth.had more effective inhibitory effects on 5 human tumor lines in vitro.Conclusion：The ursolic acid is main anticancer effective component of ethanol extraction part and trichloromethane extraction part from salvia chinensis beth.in vitro.
出处
《现代肿瘤医学》
CAS
2012年第11期2244-2248,共5页
Journal of Modern Oncology
基金
南京市卫生局重点项目(编号:ZKX09017)
关键词
石见穿
熊果酸
MTT法
体外抗肿瘤作用
salvia chinensis beth
ursolic acid
MTT assay
anticancer effect in vitro
