摘要
目的观察毫米波辐照对SD大鼠局部皮肤P物质和组织形态学的影响。方法采用40 W/cm235 GHz毫米波局部1次辐照SD大鼠30 s、1 min、3 min,测量辐照前后局部皮肤温度的变化,在辐照后不同时相点用HE染色观察局部组织形态学变化,用放射免疫法测定局部皮肤组织中P物质的变化。结果毫米波辐照大鼠后,大鼠皮肤温度显著升高;其中辐照30 s皮肤温度平均升高16.7℃,辐照1 min平均升高25.6℃,辐照3 min平均升高33.2℃。皮肤HE染色可见表皮真皮层不同程度组织形态学改变,随辐照时间的增加皮肤损伤加重,可引起胶原致密化和局部变性坏死。大鼠局部皮肤中P物质含量在辐照后第5 min和10 min显著升高,1 h后回复至对照组水平。结论在本实验条件下,毫米波辐照30 s就可致大鼠皮肤损伤和痛觉反应,这可能与其明显的热效应和P物质释放有关。
Objective To determine P and histomorphology in rat skin. Methods the effects of millimeter wave （MMW） irradiation on substance A total of 126 health adult SD rats were randomly divided into MMW irradiation group （ n = 117） and normal control group （ n = 9 ）. The outside skin on the back of MMW irradiation rats was radiated by 35 GHz MMW （40 W/cm2） for 30 s, and 1 and 3 min respectively. Rat skin temperature was detected before and after irradiation with infrared thermometer. The skin samples were taken at 0, 5 and 10 min, and 1 h after irradiation for detecting substance P （SP） level with radioimmunoassay （n = 6 for each time point）, and taken at 1, 3, 6 and 24 h after irradiation for observing histomorphology with HE staining （n = 3 for each time point）. Results MMW irradiation resulted in an obvious elevation of local skin temperature, and collagen congestion and local degeneration necrosis at different degrees in the epidermal and dermal layers of the skin. The skin injury was deteriorated with elapse of irradiation time. SP level was increased significantly at 5 and 10 min after MMW irradiation, but it returned to the level of normal control at 1 h. Conclusion Based on our experiment condition, MMW irradiation induces the rat skin damage and hyperalgesia, which may be related with markedly elevated skin temperature （thermal effect） and release of SP.
出处
《第三军医大学学报》
CAS
CSCD
北大核心
2012年第21期2163-2166,共4页
Journal of Third Military Medical University
基金
重庆市自然科学基金(CSTC2008BB5116)~~
关键词
毫米波
P物质
热效应
皮肤
millimeter wave
substance P
thermal effect
skin
