摘要
目的:定量研究急性髓系白血病(acute myeloid leukemia,AML)中BCL2L10基因启动子区异常甲基化状态,探讨其与AML的临床关系。方法:应用焦磷酸测序法,对62例原发AML患者的骨髓进行BCL2L10基因启动区甲基化水平的定量检测,并以10例缺铁性贫血和巨幼细胞性贫血的骨髓标本及10例健康人外周血标本作为对照。结果:62例AML中32例标本BCL2L10基因启动区高甲基化,阳性率为51.6%,而20例对照组中1例标本BCL2L10基因启动区高甲基化,阳性率为5.0%;BCL2L10基因在AML患者中的高甲基化阳性率显著高于对照组(P<0.01);BCL2L10基因的甲基化状态与AML患者的年龄、性别及临床分型间均未出现显著相关性(P>0.05)。结论:BCL2L10基因启动子区异常甲基化在AML中普遍存在,其可能是引起AML的分子机制之一。
Objective To quantitatively analyze the abnormal methylation in the promoter region of BCL2L10 gene in acute myeloid leukemia（AML）,and to explore the clinical relation between BCL2L10 methylation levels and AML.Methods Pyrosequencing method was applied to quantitatively examine the methylation in the promoter of BCL2L10 gene in 62 bone marrow samples from patients with primary AML.20 cases of specimens（10 cases of bone marrow specimens from patients either with iron-deficiency anemia or megaloblastic anemia,another 10 cases of peripheral blood samples from healthy persons） were as control group.Results BCL2L10 gene hypermethylation was shown in 32 samples（51.6%） among 62 samples from primary AML patients,while that was shown only in 1 sample（5.0%） among 20 controls（P 0.05）.Conclusions The abnormal methylation in the promoter region of BCL2L10 gene is a common feature in AML,which may be one of the molecular mechanisms of the pathogenesis of AML.
出处
《实用医学杂志》
CAS
北大核心
2012年第20期3397-3400,共4页
The Journal of Practical Medicine
