摘要
利用质粒pHsh为表达载体,构建高效表达N-乙酰鸟氨酸脱乙酰基酶的基因工程菌E.coli DH10B/argE-pHsh。为提高酶活并降低生产成本,优化了诱导条件。结果表明:NAOase可在pHsh系统中高活性表达,诱导起始OD600为0.6,在空气摇床中42℃热激诱导5 h重组菌比酶活达到152 U/mL。
Using pHsh as the expressing vector, the genetic engineering strain argE-pHsh producing acetylomithine deacetylase was successfully constructed. In order to improve the NAOase activity and to reduce the cost, the inducement conditions and culture medium compoments were optimized. The result showed that NAOase was over-expressed in E. coli using pHsh expression vector in fermentors with the ＂flow-in-heat＂ method. The NAOase activity reached 152 U/mL after the cells harboring pHsh-argE were heat-shocked and continued to grow at 42 ℃ for 5 h.
出处
《生物加工过程》
CAS
CSCD
2012年第5期67-71,共5页
Chinese Journal of Bioprocess Engineering
基金
国家重点基础研究发展计划(973计划)资助项目(2003CB716004)
