摘要
目的:研究油橄榄叶中橄榄苦苷超声辅助提取最佳工艺条件。方法:选定甲醇体积分数、料液比和提取时间作为影响因素,以橄榄苦苷提取率为评价指标。在单因素试验的基础上,通过3因素3水平Box-Behnken试验,建立橄榄苦苷提取率的二次多项式回归方程,经响应面回归分析得到优化组合条件。结果:最佳工艺条件为甲醇体积分数93.5%、料液比1:25(g/mL)、提取时间26min。结论:油橄榄叶中橄榄苦苷提取率可达7.83%。
Objective： To find optimal process conditions for the ultrasound-assisted extraction of oleuropein from Olea europaea leaves. Methods： The effects of methanol concentration, solid-to-solvent ratio and extraction time on oleuropein yield were investigated by one-factor-at-a-time design. A three-factor, three-level Box-Behnken experimental design was employed to build a second-order polynomial regression equation. The obtained regression equation was analyzed by response surface analysis to optimize the three process conditions. Results： The optimal process conditions were as follows： methanol concen- tration of 93.5%, solid-to-solvent ratio of 1：25 （g/mL） and an extraction time of 26 rain. Conclusion： Under these conditions, the oleuropein yield from Olea europaea leaves was up to 7.83%.
出处
《食品科学》
EI
CAS
CSCD
北大核心
2012年第18期73-76,共4页
Food Science
关键词
油橄榄叶
橄榄苦苷
超声提取
响应面分析
Olea europaea leaves
oleuropein
ultrasound-assisted extraction~ response surface methodology
