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合成生物学的关键技术及应用进展 被引量：6

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摘要 20世纪的生物学研究一直着眼于对生物系统的不断分解，解剖至细胞中单个蛋白或基因，研究其结构和功能来解释生命现象。但随着当代分子生物学技术的迅猛发展，以系统化设计和工程化构建为理念的合成生物学成为新一代生物学的发展方向。
作者 邢玉华 谭俊杰 李玉霞 凌焱 刘刚 陈惠鹏
机构地区 军事医学科学院生物工程研究所 吉林大学生命科学学院
出处 《中国医药生物技术》 CSCD 2012年第5期357-363,共7页 Chinese Medicinal Biotechnology
基金 国家高技术研究发展计划(863计划)子课题(2012AA022001-03D)
关键词 合成生物学 分子生物学技术 应用 生物系统 生命现象 工程化 系统化
分类号 R914 [医药卫生—药物化学]
  • 相关文献

参考文献37

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  • 2Gibson DG, Benders GA, Andrews-Pfannkoch C, et al. Complete chemical synthesis, assembly, and cloning of a Mycoplasma genitalium genome. Science, 2008, 319(5867): 1215-1220.
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二级参考文献10

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共引文献4

同被引文献92

  • 1凌焱,段海清,陈惠鹏.合成生物学[J].军事医学科学院院刊,2006,30(6):572-574. 被引量：13
  • 2BalanceDJ.重组人生长激素和人血清白蛋白融合蛋白[P].CN1207131.1999.
  • 3Cello J, Paul AV, Wimmer E. Chemical synthesis of poliovirus cDNA: generation of infectious virus in the absence of natural template. Science, 2002, 297(5583): 1016-8.
  • 4Smith HO, Hutchison CA 3rd, Pfannkoch C, et al. Generating a synthetic genome by whole genome assembly: phiX174 bacteriophage from synthetic oligonucleotides. Proc Natl Acad Sci USA, 2003, 100(26): 15440-5.
  • 5Lartigue C, Glass JI, Alperovich N, et al. Genome transplantation in bacteria: changing one species to another. Science, 2007, 317(5838): 632-8.
  • 6Gibson DG, Benders GA, Andrews PC, et al. Complete chemical synthesis, assembly, and cloning of a Mycoplasma genitalium genome. Science, 2008, 319(5867): 1215-20.
  • 7Gibson DG, Glass JI, Lartigue C, et al. Creation of a bacterial cell controlled by a chemically synthesized genome. Science, 2010, 329(5987): 52-6.
  • 8Gibson DG, Young L, Chuang RY, et al. Enzymatic assembly of DNA molecules up to several hundred kilobases. Nat Methods, 2009, 6(5): 343-5.
  • 9Dymond JS, Richardson SM, Coombes CE, et al. Synthetic chromosome arms function in yeast and generate phenotypic diversity by design. Nature, 2011, 477(7365): 471-6.
  • 10Prodromou C, Pearl LH. Recursive PCR: a novel technique for total gene synthesis. Protein Eng, 1992,5(8): 827-9.

引证文献6

二级引证文献9

中国医药生物技术
2012年 第5期

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