摘要
研究Pb对斑马鱼抗氧化酶活性影响,探讨重金属Pb对斑马鱼毒理学效应。以斑马鱼为模式生物,采用半静态法对斑马鱼进行驯养和试验,测定不同浓度Pb对超氧化物歧化酶(SOD)和过氧化氢酶(CAT)活性影响。Pb暴露1d胁迫斑马鱼SOD为诱导效应,胁迫CAT为诱导-抑制效应,Pb暴露7 d胁迫斑马鱼SOD为诱导效应,胁迫CAT为诱导-抑制效应,且暴露1 d和暴露7 d酶活性变化均显著。试验结果表明,Pb胁迫斑马鱼抗氧化系统酶活性变化显著,Pb毒性显著。结果为重金属污染对水生生物毒性机理研究提供参考。
The effect of Pb on the anti-oxidative enzyme activities in Danio rerio was studied, and the toxicity effect of Pb on Danio rerio was discussed. Danio rerio was viewed as model organism, semi-static toxicity test was used in domestication and experiments of Danio rerio, the effects of Pb on superoxide dismutase （SOD） and eatalase （CAT） of it were discussed. At 1 d, effects of Pb stress on SOD in Danio rerio was induction effect, and the stress on CAT was induction-inhibition effect. At 7d, effects of PbAc stress on SOD in Danio rerio was induction effect, and stress on CAT was induction-inhibition effect. Moreover, the enzyme activities were changed markedly significantly for 1 or 7d. The results showed that Pb stress on the anti-oxidative enzyme activities in Danio rerio was changed markedly. In short time and low-concentration exposure, Pb toxicity was significantly. The study could provide references for heavy metal pollution on the aquatic organism toxicity mechanism.
出处
《环境科技》
2012年第5期1-4,共4页
Environmental Science and Technology
基金
国家环保公益性科研项目(201009041)
安徽省自然科学基金项目(1208085MB26)
安徽省环境科学研究院院长基金项目(2010001)
