摘要
为了研究从浙江省温州市南麂岛近海海域海洋沉积物样品中分离得到的一株具有抗大黄鱼病原菌活性的真菌NJ0104的分类地位,在标准培养条件下,采用传统形态鉴定方法,结合ITS-5.8S rDNA片段PCR扩增和序列测定,建立系统发育树。形态学特征显示菌丝呈树枝状分支,分支较多,无核,有隔膜,瓶体式产孢;ITS序列进行测序全长为1 027 bp。结合形态学特征和GenBank中核酸数据比对分析。结果表明,菌株NJ0104与节菱孢属亲缘关系较近,具有98%以上的相似性,因此将其鉴定为节菱孢属(Arthrinium sp.)。
A marine fungal strain NJ0104, isolated from marine sediments near Nanji island sea, Wenzhou, Zhe- jiang, could produce active metabolites against bacterial pathogen of Pseudoseiaena. To identify this strain, morphological and ITS-5.8S rDNA regions of the strain was amplified through PCR reaction and sequenced. Mycelium showed a polybranch morphology, there was no nucleus＇, but septum were present in the myeelium and produced eonidiospores. The length of ITS sequence was 1027 bp. The results of morphological observation and genetic relationship of strain NJ0104 was close to Arthinium and the similarity was more than 98%. Based upon the combination of phenotypie and genotypie data, the strain was identified as Arthrinium sp.
出处
《浙江农业学报》
CSCD
北大核心
2012年第5期801-807,共7页
Acta Agriculturae Zhejiangensis
基金
浙江省科技厅面上项目(2009C33004)
温州市科技计划资助项目(Y20080092)
关键词
抗大黄鱼病原菌
抑菌活性
形态学
ITS序列
NJ系统发育树
anti-microbial pathogen of Pseudoseiaena
antibacterial activity
morphological characteristics
ITS sequences
NJ phylogeny tree
