摘要
目的:建立测定尿液中巴比妥类药物水平[巴比妥(Barbital,bar)、苯巴比妥(Phenobarbital,phe)、异戊巴比妥(Amobarbital,amo)、司可巴比妥(Secobarbital,sec)]的高灵敏度的高效液相色谱-串联质谱联用(HPLC-MS/MS)分析方法。方法:尿液经乙醚+环己烷(1∶1)液液萃取、氮气吹干浓缩后,用高效液相色谱-串联质谱联用仪分析。结果:尿液中4种巴比妥类药物在0.01 mg/L～0.2 mg/L浓度范围内呈良好线性,线性相关系数r均大于0.99;加标回收率为91.3%～106.8%;bar的方法定量检出限为0.005 mg/L;amo、sec、phe的方法定量检出限为0.002 mg/L。结论:该方法检出限较低、线性范围宽、分析时间较短,可用于检测人体尿液中巴比妥类药物的含量。
Objective：To establish the high-performance liquid chromatography tandem mass spectrometry（HPLC-MS/MS） with high sensitivity and lower detection for the determination of barbiturates [barbital（bar）,phenobarbital（phe）,amobarbital（amo） and secobarbital（sec）] in urine.Methods： The human urine was processed by liquid-liquid extraction（LLE）,concentrated by nitrogen and analyzed by HP LC-MS/MS.Results： 4 kinds of barbiturates in urine showed good linearity in the concentration range of 0.01 mg/L^0.2 mg/L and the linear correlation coefficient was over 0.99.The recovery was between 91.3% and 106.8%.The detection limit of bar was 0.005 mg/L.The limit of detection of phe,amo and sec were all 0.002 mg/L.Conclusion： This method can be used for determination of barbital in human urine with wide linear range,short analytical time and low detection limit.
出处
《中国卫生检验杂志》
北大核心
2012年第9期2003-2006,共4页
Chinese Journal of Health Laboratory Technology
基金
江苏省预防医学科研课题(Y201022)
无锡市卫生系统科研项目(ZD1009)
