摘要
目的探讨兔肝VX-2瘤种植后磁共振扩散成像ADC值动态特征。方法新西兰大白兔40只，采用开腹下块种植方法建立肝内VX-2瘤模型47只（25只种植1个部位、15只种植2个部位）。对全部种植兔于种植后第7、14、21天行DWI并对部分肿瘤标本行病理检查。采用SPSS12．0对不同时间、不同部位、不同b值组之间ADC值进行统计分析。结果（1）种植后第7、14与21天扫描，b值为100sdmm2，47例瘤周正常肝ADC值、肿瘤周围、肿瘤中央ADC值均高于b值为300s／mm。（F=17．964，P〈0．01；F=13．986，P〈0．01；F=128．681，P〈0．01），不同b值时各部位ADC值差异均有统计学意义。（2）b值为100或300s／mm2时，种植后相同时间扫描正常肝实质ADC值高于肿瘤周围部分与肿瘤中央部分（F=176．586，P〈0．01；F=55．089，P〈0．01），不同部位相同b值ADC值差异有统计学意义。（3）种植后第7、14与21天，肿瘤周围ADC值与肿瘤中央ADC值逐渐降低（b=100s／mm2，F=48．211，P〈0．01；b=300s／mm2，F=20．955，P〈0．01），不同时间各部位ADC值差异均有统计学意义。（4）种植后第7天与14天肿瘤未见明显坏死区，但是存在水肿等因素影响导致肿瘤ADC值下降部不明显。21d时肿瘤内部出现明显坏死，成活肿瘤部分ADC值明显低于正常肝实质，肿瘤坏死部分ADC值高低不等，而囊变、液化部分ADC值可高于正常肝实质。结论ADC值可以客观地反映肿瘤内部水分子的扩散情况与了解肿瘤的生长与坏死液化情况，在动态监视肿瘤的生长情况与评价肿瘤的恶性程度及疗效等方面有重要的潜在价值。
Objective To. investigate dynamically characteristics of apparent diffusion coefficient （ADC） of MR diffusion-weighted imaging （DWI） in the rabbit VX-2 tumor model. Methods Forty New Zealand rabbits were included in the study and forty-seven rabbit VX-2 tumor models were raised by implan- ting directly and intrahepatically after abdominal cavity was opened. DWI was carried out periodically and respectively on seventh, fourteenth, and twenty-first day after implantation. Part samples of VX-2 tumors were studied by pathology. The distinction of VX-2 tumors on DWI was assessed by their ADC values. The statistical significance between different time groups, different area groups, or different b-value groups was calculated using SPSS12. 0 software, respectively. Results ADC values of 47 VX-2 tumors in the area of tumor periphery, tumor center, and normal parenchyma around tumor were greater when b-value was 100 s/ mm2 than those when b-value was 300 s/mm2 and the distinction of VX-2 tumor ADC in the area of tumor periphery, tumor center, and normal parenchyma around tumor between different b-value groups was signifi- cant, respectively（ F =17.964, P 〈0.01; F =13.986, P 〈0.01; F =128.681, P 〈0.01）. The ADC values in the area of normal liver parenchyma around tumor were greater than those in the area of VX-2 tum-or periphery and tumor center when the b-value was 100 or 300 s/ram2. When b-value was the same（ 100 or 300 s/mm2 ）, the distinction of VX-2 tumor ADC between different areas was significant（ F = 176. 586, P 〈 0. 01 ; F = 55. 089, P 〈 0. 01 ）. The ADC of VX-2 tumor in the area of tumor periphery and tumor center be- came gradually low from seventh to fourteenth or twenty-first day after implantation and the distinction of ADC between different time groups but the area same （？） was significant（b = 100 s/ram2, F =48. 211, P 〈0. 01 ; b =300 s/mm2, F =20. 955, P 〈0. 01 ）. There were not obvious cellular necrosis in VX-2 tumors on seventh and fourteenth day after implantation but ADC of VX-2 tumor decreased unobviously because of cellular ede- mata in or around tumors. There were obvious cellular necrotic areas in VX-2 tumors on the twenty-first day after implantation. ADC of viable tumor cells in VX-2 tumors were lower on DWI than that in the area of nor- mal liver parenchyma around tumor and ADC of dead tumor cells in VX-2 tumors were unequal, including high values, equal values, and low values but they were higher than that in the area of normal liver parenchy- ma around tumor after dead tumor cells had been liquified or had become cystic. Conclusions ADC is able to reflect objectively the diffusion of water molecules in the tumor and to reflect indirectly the degree of the growth and liquified necrosis of a tumor. ADC has an important and potential value in monitoring dynamical tumor growth and in evaluating malignant degree and therapeutic effect.
出处
《中国医师杂志》
CAS
2012年第8期1022-1026,共5页
Journal of Chinese Physician
基金
湖南省自然科学基金（07JJ4022）、湖南省科技局（2012SK3066）和长沙市科技局（K1005023-31,1104054-21）
关键词
肝肿瘤
实验性/诊断
磁共振成像
弥散
兔
Liver neoplasms, experimental/diagnosis
Diffusion magnetic resonance imaging
Rabbits
