摘要
以‘金亮’锦带(Weigela florida‘Goldrush’)和‘宝石’锦带(Weigela florida‘Ruby’)为研究对象,利用20%聚乙二醇-6000(PEG-6000)人工模拟水分胁迫环境,设置4个胁迫梯度(持续0、6、12、24 h),研究在胁迫过程中植物叶片的相对含水量、细胞膜透性、丙二醛质量摩尔浓度、蛋白质和可溶性糖质量分数的变化。结果表明:经过PEG-6000处理后,随着胁迫时间的增大,叶片电导率呈上升趋势,叶片相对含水量均呈逐渐下降的趋势;‘金亮’锦带蛋白质质量分数呈现先升高后降低的趋势,而‘宝石’锦带则呈现先降低后升高最后又降低的趋势;‘金亮’锦带可溶性糖质量分数则呈逐渐升高的变化趋势,‘宝石’锦带可溶性糖质量分数则均呈先升高后降低的趋势,‘宝石’丙二醛质量摩尔浓度则均呈现先降低后升高的趋势。
An experiment was conducted to study the physiological changes of Weigela florida ' Goldrush' and Weigela florida 'Ruby' treated with 20% PEG-6000 under different stress durations (0, 12, 24, 36 h). The effect of drought stress on the two cultivars was evaluated by measuring relative water content, relative conductivity, and contents of soluble protein, malondialdehyde (MDA) and soluble sugar in the leaves. Results showed that, with increasing stress duration, the relative conductivity in the leaves of the two cultivars increased, while relative water content decreased gradually. The content of soluble protein in W. florida ' Goldrush' decreased after an increase, while that of soluble protein in W. florida ' Ru- by' decreased firstly, and then increased and kept decreased finally. The content of soluble sugar in W. florida ' Goldrush' increased, and that of soluble sugar in W. florida ' Ruby' increased firstly, and then decreased. The content of malondialdehyde (MDA) in W. florida ' Goldrush' increased after a decrease.
出处
《东北林业大学学报》
CAS
CSCD
北大核心
2012年第9期22-24,共3页
Journal of Northeast Forestry University